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Cattle in Africa are a genetically diverse population that has resulted from successive introduction of Asian Bos indicus and European B. Taurus cattle. However, analysis of mitochondrial genetic diversity in African cattle identified three lineages, one associated with Asian B. indicus, one with...


Ballingall, K.T.Waibochi, L.Homes, E.C.Woelk, C.H.MacHugh, Niall D.Lutje, V.McKeever, Declan J.[The CD45 locus in cattle: Allelic polymorphism and evidence for exceptional positive natural selection]The CD45 locus in cattle: Allelic polymorphism and evidence for exceptional positive natural selection

The mechanisms whereby trypanotolerant N'Dama cattle control infection with Trypanosoma congolense are unknown. Previous studies have suggested that the monocytes of N'Dama cattle are more highly activated during infection than those of trypanosus ceptible Boan cattle. However, we have recently r...


Taylor, K.Mertens, B.Lutje, V.Saya, R.[Trypanosoma congolense infection of trypanotolerant N'Dama (Bos taurus) cattle is associated with decreased secretion of nitric oxide by interferon-gamma-activated monocytes and increased transcription of interleukin-10]Trypanosoma congolense infection of trypanotolerant N'Dama (Bos taurus) cattle is associated with decreased secretion of nitric oxide by interferon-gamma-activated monocytes and increased transcription of interleukin-10

Memory T- and B-cell responses to trypanosome antigens were measured in peripheral blood mononuclear cells, spleen and lymph node cells obtained from 4 trypanotolerant N'Dama cattle which had been exposed to 6 experimental infections with Trypanosoma congolense. These cattle were treated with try...


Lutje, V.Taylor, K.A.Boulange, A.F.Authié, E.[Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle]Trypanosoma congolense: Tissue distribution of long-term T- and B-cell responses in cattle
Taylor, K.A.Gichuki, B.Lutje, V.Naessens, JanWilliams, D.J.L.[In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle]In vitro activation and detection of antibody-secreting cells from Trypanosoma congolense-infected cattle
Lutje, V.Black, S.J.[Analysis of pokeweed mitogen-induced in vitro proliferation and antibody responses of bovine lymphocytes]Analysis of pokeweed mitogen-induced in vitro proliferation and antibody responses of bovine lymphocytes
Lutje, V.Black, S.J.[Cellular interactions regulating the in vitro response of bovine lymphocytes to ovalbumin]Cellular interactions regulating the in vitro response of bovine lymphocytes to ovalbumin

Infection with African trypanosomes causes the diseases sleeping sickness in humans and nagana in cattle in sub-Saharan Africa. Suppression of cellular immune responses is a feature of trypanosomiasis in bovine, human, and murine hosts. Some aspects of immunosuppression in the murine model are me...


Taylor, K.Lutje, V.Mertens, B.[Nitric Oxide synthesis is depressed in Bos indicus cattle infected with Trypanosoma congolense and Trypanosoma vivax and does not mediate T-cell suppression]Nitric Oxide synthesis is depressed in Bos indicus cattle infected with Trypanosoma congolense and Trypanosoma vivax and does not mediate T-cell suppression

Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in ...


Taylor, K.A.Lutje, V.Kennedy, D.Authié, E.Boulange, A.F.Logan-Henfrey, L.L.Gichuki, B.Gettinby, G.[Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle]Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle

A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmitted Trypanosoma congolense was conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferat...


Lutje, V.Taylor, K.A.Kennedy, D.Authié, E.Boulange, A.F.Gettinby, G.[Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection]Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection

T-cell-mediated immune responses to defined antigens of Trypanosoma congolense were measured in cattle undergoing primary infection. The antigens used were the variable surface glycoprotein and two invariant antigens, a 33-kDa cysteine protease (congopain) and a recombinant form of a 69-kDa heat-...


Lutje, V.Mertens, B.Boulange, A.F.Williams, D.J.L.Authié, E.[Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle]Trypanosoma congolense: Proliferative responses and interleukin production in lymph node cells of infected cattle

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