It is prerequisite to extract Ribonucleic Acid (RNA) with high quality and integrity in order to carry out molecular biology studies in any plant species. Samples collected from remote fields require preservation before being processed for RNA extraction and downstream process like RT-PCR, real t...
Munguti, F.M.
,
Kilalo, D.C.
,
Macharia, M.
,
Magiri, E.N.
,
Kinyua, J.K.
,
Holton, T.A.
,
[Evaluation of Passiflora edulis leaf sample storage methods on RNA quality and suitability for use in RT-PCR assays]
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Evaluation of Passiflora edulis leaf sample storage methods on RNA quality and suitability for use in RT-PCR assays
Root necrosis similar to those of the cassava brown streak disease (CBSD) were observed on cassava in western provinces of the Democratic Republic of Congo (DR.Congo) in the early 2000’s. However molecular laboratory diagnosis were not able to detect any causative agent responsible for the attack...
Bakelana, Z.
,
Magembe, E.
,
Laura, M.B.
,
Macharia, M.
,
Mahungu, N.
,
Tata-Hangy, W.
,
Lutete, D.
,
Monde, G.
,
Harvey, J.
,
Ndunguru, J.
,
Kayuki, C.
,
Pita, J.
,
Lema, K.M.
,
Kanana, T.
,
[Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo]
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Attempts to identify Cassava Brown Streak Virus in western Democratic Republic of Congo
Aims: To optimize the different available molecular based techniques, validate the Loop-mediated isothermal amplification (LAMP) assay for detection of Capripoxviruses (CaPVs) DNA and deep sequencing to confirm the results of the LAMP assay and ensure that an effective and reliable detection meth...
Omoga, D.C.A.
,
Macharia, M.
,
Magiri, E.
,
Kinyua, J.
,
Kasiiti, J.
,
Holton, T.
,
[Molecular based detection, validation of a LAMP assay and phylogenetic analysis of Capripoxvirus in Kenya]
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Molecular based detection, validation of a LAMP assay and phylogenetic analysis of Capripoxvirus in Kenya
Dasheen mosaic virus (DsMV) is member of the family Potyviridae, genus Potyvirus and infects taro and other aroids wherever they grow. DsMV infection results in characteristic feathery-mottle and mosaic symptoms on leaves and can cause up to 60% production losses on taro and other edible aroids (...
Kidanemariam, D.B.
,
Macharia, M.
,
Harvey, Jagger J.W.
,
Holton, T.
,
Sukal, A.
,
James, A.P.
,
Harding, R.M.
,
Abraham, A.
,
[First report of Dasheen mosaic virus infecting taro (Colocasia esculenta L.) from Ethiopia]
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First report of Dasheen mosaic virus infecting taro (Colocasia esculenta L.) from Ethiopia