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Autor: Machuka, J.

Crops' incompatibility makes conventional breeding approaches untenable in transferring available insect resistance From wild Vigna sp. into cowpea. The alternative recourse is to isolate and transfer alien resistance genes using genetic transformation. This has the added advantage of using usefu...


Machuka, J.[Potential role of transgenic approaches in the control of cowpea insect pests]Potential role of transgenic approaches in the control of cowpea insect pests

Over the last three decades, sporadic efforts have been made to develop regeneration and transformation systems in cowpea ( Vigna unguiculata L. Walp). This paper reviews the progress made to date, including highlights of culture media and explants used for regeneration and chimeric gene construc...


Machuka, J.Adesoye, A.Obembe, O.O.[Regeneration and genetic transformation in cowpea]Regeneration and genetic transformation in cowpea

Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose...


Machuka, J.Okeola, O.[One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins]One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins

The legume pod‐borer Maruca vitrata (Fabricius), [Lepidoptera: Pyralidae] is a major constraint restricting increased cowpea production in tropical Africa and Asia. Since lectins are known to have insecticidal properties against several pests, a survey was undertaken to screen for the effects of ...


Machuka, J.Damme, E.V.Peumans, W.J.Jackai, L.E.N.[Effect of plant lectins on larval development of legume pod borer, Maruca vitrata]Effect of plant lectins on larval development of legume pod borer, Maruca vitrata

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