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The cowpea beetle, Callosobruchus maculatus, and pod-sucking bug, Clavigralla tomentosicollis, are two of the major insect pests of cowpea in Africa. A lectin was purified from the seeds of the African yam bean (AYB), Sphenostylis stenocarpa, by affinity chromatography on Galactosc-Sepharose 4B. ...


Okeola, O.G.Machuka, J.Fasidi, I.O.[Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug]Insecticidal activities of the African yam bean seed lectin on the development of the cowpea beetle and the pod sucking bug
Autor: Machuka, J.

Crops' incompatibility makes conventional breeding approaches untenable in transferring available insect resistance From wild Vigna sp. into cowpea. The alternative recourse is to isolate and transfer alien resistance genes using genetic transformation. This has the added advantage of using usefu...


Machuka, J.[Potential role of transgenic approaches in the control of cowpea insect pests]Potential role of transgenic approaches in the control of cowpea insect pests

Over the last three decades, sporadic efforts have been made to develop regeneration and transformation systems in cowpea ( Vigna unguiculata L. Walp). This paper reviews the progress made to date, including highlights of culture media and explants used for regeneration and chimeric gene construc...


Machuka, J.Adesoye, A.Obembe, O.O.[Regeneration and genetic transformation in cowpea]Regeneration and genetic transformation in cowpea

Sweetpotato (Ipomoea batatas) is one of the most important food crops in tropical and subtropical countries. In Sub-Saharan Africa, sweetpotato is mainly produced for consumption and as a source of income by resource-poor farmers. However, their production is limited by severe damage caused by pe...


Manrique Trujillo, S.Wamalwa, N.L.Díaz, D.Reano, R.Tovar, J.C.Prentice, K.Ormachea, M.Kreuze, J.F.Machuka, J.Ghislain, M.[Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.]Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.
Ghislain, M.Tovar, J.Prentice, K.Ormachea, M.Rivera, C.Manrique, S.Kreuze, J.F.Ssemakula, G.N.Rukarwa, R.Sefasi, A.Mukasa, S.Wamalwa, L.Machuka, J.[Weevil resistant sweetpotato through biotechnology.]Weevil resistant sweetpotato through biotechnology.

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