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Mihok, S.Munyoki, E.Brett, R.A.Jonyo, J.F.Rottcher, D.Majiwa, P.A.O.Kang'ethe, Erastus K.Kaburia, H.F.A.Zweygarth, E.[Trypanosomiasis and the conservation of black rhinoceros (Diceros bicornis) at the Ngulia Rhino Sanctuary, Tsavo West National Park, Kenya]Trypanosomiasis and the conservation of black rhinoceros (Diceros bicornis) at the Ngulia Rhino Sanctuary, Tsavo West National Park, Kenya

Blood samples were collected from parasitaemic cattle in the Ghibe Valley, Ethiopia, frozen in liquid nitrogen and transported to Nairobi, Kenya. Twelve of the stabilates were inoculated into individual Boran (Bos indicus) calves and characterised for their sensitivity, in turn, to diminazene ace...


Codjia, V.Mulatu, W.Majiwa, P.A.O.Leak, S.G.A.Rowlands, G.J.Authié, E.D'Ieteren, G.D.M.Peregrine, A.S.[Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium]Epidemiology of bovine trypanosomiasis in the Ghibe valley, southwest Ethiopia. 3. Occurrence of population of Trypanosoma congolense resistant to diminazene, isometamidium and homidium

During its first two years, the African trypanosome genome project, funded in part by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), has concentrated on three fundamental areas: (1) gene discovery by single-pass sequencing of cDNAs to produce expre...


Melville, S.E.Majiwa, P.A.O.Donelson, J.E.[Resources available from the African Trypanosome Genome Project]Resources available from the African Trypanosome Genome Project

The pathogenic trypanosomes Trypanosoma vivax and Trypanosoma congolense are transmitted by tsetse and cause trypanosomosis, a debilitating disease affecting livestock in Africa and South America. Here, we discuss the need, relevance and advantage of having the Trypanosoma genomes sequenced, an...


Davila, A.M.R.Majiwa, P.A.O.Grisard, E.C.Aksoy, S.Melville, S.E.[Comparative genomics to uncover the secrets of tsetse and livestock-infective trypanosomes]Comparative genomics to uncover the secrets of tsetse and livestock-infective trypanosomes

A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...


Masake, R.A.Ole-MoiYoi, O.K.Urakawa, T.Hirumi, H.Majiwa, P.A.O.Wells, C.W.Minja, S.H.Makau, J.M.Nantulya, V.M.[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then ...


Agbo, E.C.Clausen, P.H.Buscher, P.Majiwa, P.A.O.Claassen, E.Marinus, F.W.P.[Population genetic structure and cladistic analysis of Trypanosoma brucei isolates]Population genetic structure and cladistic analysis of Trypanosoma brucei isolates

The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. All T. evansi isolates hitherto tested express this VAT. In a study on the differential diagnosis of T. equiperdum and T. evansi in horses, we investigated serolo...


Claes, F.Verloo, D.Waal, D.T. deUrakawa, T.Majiwa, P.A.O.Goddeeris, B.M.Buscher, P.[Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum]Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum

Trypanosomosis remains one of the biggest impediments to the Development of the livestock industry in sub-Saharan Africa while it is likely that a vaccine for this disease could be found in the future, none is presently available. Mean-while different strategies are being followed to control this...


Majiwa, P.A.O.Konde, V.Souza, M. dePeregrine, A.S.Maina, M.[Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense]Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense

Studies on infections with drug-resistant trypanosomes indicate that the majority of parasites in a population are sensitive to the drug dose used to select the population (Mamman et al., 1993). This study was initiated to examine whether a drug-resistant trypanosome population could influence th...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Murphy, N.B.[Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction]Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction

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