In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T...
Claes, F.
,
Verloo, D.
,
Waal, D.T. de
,
Majiwa, P.A.O.
,
Baltz, T.
,
Goddeeris, B.M.
,
Buscher, P.
,
[The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum]
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The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum
The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...
Lejon, Veerle
,
Claes, F.
,
Verloo, D.
,
Maina, M.
,
Urakawa, T.
,
Majiwa, P.A.O.
,
Buscher, P.
,
[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]
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Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels
A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T. evansi infection in livestock, was cloned as a recombinant plasmid and sequenced. A recombinant baculov...
Urakawa, T.
,
Verloo, D.
,
Moens, L.
,
Buscher, P.
,
Majiwa, P.A.O.
,
[Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2]
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Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2
To clarify evolution and phylogenetic relationships of trypanosome alternative oxidase (AOX) molecules, AOX genes (cDNAs) of the African trypanosomes, Trypanosoma congolense and Trypanosoma evansi, were cloned by PCR. Both AOXs possess conserved consensus motifs (-E-, -EXXH-). The putative amino ...
Suzuki, T.
,
Hashimoto, T.
,
Yabu, Y.
,
Majiwa, P.A.O.
,
Ohshima, S.
,
Suzuki, M.
,
Lu, S.
,
Hato, M.
,
Kido, Y.
,
Sakamoto, K.
,
Nakamura, K.
,
Kita, K.
,
Ohta, N.
,
[Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families]
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Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families