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The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...


Lejon, VeerleClaes, F.Verloo, D.Maina, M.Urakawa, T.Majiwa, P.A.O.Buscher, P.[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels

Polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) probes were used to characterize trypanosomes from cattle in Morogoro region of Tanzania. Blood samples collected from 390 beef and dairy cattle in selected farms in Morogoro region were examined for presence of trypanosomes using th...


Mugittu, K.N.Silayo, R.S.Majiwa, P.A.O.Kimbita, E.K.Mutayoba, B.M.MaSelle, R.[Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania]Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania

Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...


Masake, R.A.Njuguna, J.T.Brown, C.C.Majiwa, P.A.O.[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock
Majiwa, P.A.O.Young, J.R.Hamers, R.Mathyssens, G.[Minichromosomal variable surface glycoprotein genes and molecular karyotypes of Trypanosoma (Nannomonas) congolense]Minichromosomal variable surface glycoprotein genes and molecular karyotypes of Trypanosoma (Nannomonas) congolense
Williams, R.O.Young, J.R.Majiwa, P.A.O.[Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei]Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei
Young, J.R.Donelson, J.E.Majiwa, P.A.O.Shapiro, S.Z.Williams, R.O.[Analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei]Analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei

A study was carried out to determine whether a drug-resistant trypanosome population could influence the survival of a drug-sensitive population in mixed infections in goats. To identify both populations during the course of a mixed infection, a system for distinguishing them was developed; using...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Mbiuki, S.M.Murphy, N.B.[Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats]Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats

There have been tremendous advances in our knowledge of trypanosome biology, yet many aspects remain unclear. Currently, the genome of Trypanosome brucei is being sequenced and this, with other genome-wide analysis methods, could provide novel insights into the parasite and facilitate the develop...


Agbo, E.C.Majiwa, P.A.O.Bascher, P.Claassen, E.Marinus, F.W.P.[Trypanosoma brucei genomics and the challenge of identifying drug and vaccine targets]Trypanosoma brucei genomics and the challenge of identifying drug and vaccine targets
Majiwa, P.A.O.Masake, R.A.Nantulya, V.M.Hamers, R.Matthyssens, G.[Trypanosoma (Nannomonas) congolense. Identification of two karyotypic groups]Trypanosoma (Nannomonas) congolense. Identification of two karyotypic groups

Ten trypanosome isolates were collected at random from cattle at Ghibe, Ethiopia, in February 1993 and all shown to be savannah-type Trypanosoma congolense. When inoculated into naive Boran (Bos indicus) calves, all 10 isolates were resistant to diminazene aceturate (Berenil), isometamidium chlor...


Mulugeta, W.Wilkes, J.Mulatu, W.Majiwa, P.A.O.Masake, R.Peregrine, A.S.[Long-term occurrence of Trypanosoma congolense resistant to diminazene, isometamidium and homidium in cattle at Ghibe, Ethiopia]Long-term occurrence of Trypanosoma congolense resistant to diminazene, isometamidium and homidium in cattle at Ghibe, Ethiopia

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