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Isometamidium chloride has remained a very important prophylactic and therapeutic drug against trypanosomosis in cattle since its introduction into the market in the 1950s with, unfortunately, a concomitant development of resistance in trypanosomosis endemic areas. Amplified Fragment Length Polym...


Delespaux, V.Geysen, D.Majiwa, P.A.O.Geerts, S.[Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense]Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense

Polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) probes were used to characterize trypanosomes from cattle in Morogoro region of Tanzania. Blood samples collected from 390 beef and dairy cattle in selected farms in Morogoro region were examined for presence of trypanosomes using th...


Mugittu, K.N.Silayo, R.S.Majiwa, P.A.O.Kimbita, E.K.Mutayoba, B.M.MaSelle, R.[Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania]Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania

Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...


Masake, R.A.Njuguna, J.T.Brown, C.C.Majiwa, P.A.O.[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock

The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...


Masake, R.A.Majiwa, P.A.O.Moloo, S.K.Makau, J.M.Njuguna, J.Maina, M.Kabata, J.M.Ole-MoiYoi, O.K.Nantulya, V.M.[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

We have evaluated whether sequence polymorphisms in the rRNA intergenic spacer region can be used to study the relatedness c.' two subspecies of Trypanosoma brucei. Thirteen T. bnrcei isolates made up of 6 T. b. brucei and 7 T. b. gambiense were analyzed using restriction fragment length polymorp...


Agbo, E.C.Majiwa, P.A.O.Classsen, E.J.H.M.Roos, M.H.[Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization]Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization

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