In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T...
Claes, F.
,
Verloo, D.
,
Waal, D.T. de
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Majiwa, P.A.O.
,
Baltz, T.
,
Goddeeris, B.M.
,
Buscher, P.
,
[The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum]
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The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum
The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...
Lejon, Veerle
,
Claes, F.
,
Verloo, D.
,
Maina, M.
,
Urakawa, T.
,
Majiwa, P.A.O.
,
Buscher, P.
,
[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]
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Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels
Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...
Masake, R.A.
,
Njuguna, J.T.
,
Brown, C.C.
,
Majiwa, P.A.O.
,
[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]
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The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock
The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...
Masake, R.A.
,
Majiwa, P.A.O.
,
Moloo, S.K.
,
Makau, J.M.
,
Njuguna, J.
,
Maina, M.
,
Kabata, J.M.
,
Ole-MoiYoi, O.K.
,
Nantulya, V.M.
,
[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]
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Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction