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Majiwa, P.A.O.Webster, P.[A repetitive deoxyribonucleic acid sequence that distinguishes Trypanosoma simiae from T. congolense]A repetitive deoxyribonucleic acid sequence that distinguishes Trypanosoma simiae from T. congolense

Transcripts which encode two metacyclic-form-specific variable surface glycoproteins (mVSGs) of Trypanosoma congolense IL3000 have been cloned into baculovirus expression vectors using a novel transfer vector, pAcL11. One of the recombinant baculoviruses (AcVSG1) expressed a mVSG as a glycoprotei...


Urakawa, T.Eshita, Y.Fukuma, T.Hirumi, H.Hirumi, K.Majiwa, P.A.O.[Expression of Trypanosoma congolense antigens in spodoptera frugiperdia insect cells]Expression of Trypanosoma congolense antigens in spodoptera frugiperdia insect cells

The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...


Masake, R.A.Majiwa, P.A.O.Moloo, S.K.Makau, J.M.Njuguna, J.Maina, M.Kabata, J.M.Ole-MoiYoi, O.K.Nantulya, V.M.[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

We have evaluated whether sequence polymorphisms in the rRNA intergenic spacer region can be used to study the relatedness c.' two subspecies of Trypanosoma brucei. Thirteen T. bnrcei isolates made up of 6 T. b. brucei and 7 T. b. gambiense were analyzed using restriction fragment length polymorp...


Agbo, E.C.Majiwa, P.A.O.Classsen, E.J.H.M.Roos, M.H.[Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization]Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization

A bobine cDNA encoding erythropoietin (Epo) was isolated by polymerase chain reaction (PCR) amplification and screening of a bovine kidney cDNA library. The sequenced cDNA has a length of 1312 bp and an open reading frame that encodes a predicted 192-amino-acid (aa) protein, including a putative ...


Suliman, H.B.Majiwa, P.A.O.Feldman, B.F.Mertens, B.Logan-Henfrey, L.L.[Cloning of a cDNA encoding bovine erythropoietin and analysis of its transcription in selected tissues]Cloning of a cDNA encoding bovine erythropoietin and analysis of its transcription in selected tissues
Nyeko, J.H.P.Ole-MoiYoi, O.K.Majiwa, P.A.O.Otieno, L.H.Ociba, P.M.[Characterization of Trypanosoma isolates from cattle in Uganda using species-specific DNA probes reveals predominace of mixed infection]Characterization of Trypanosoma isolates from cattle in Uganda using species-specific DNA probes reveals predominace of mixed infection

The recent genome initiatives of the neglected parasites Trypanosoma vivax and Trypanosoma congolense, and the vector tsetse are promising with regards to the data available in public databases. While these genome initiatives and the potential for developing new drugs and vaccines are admirable, ...


Davila, A.M.R.Majiwa, P.A.O.Grisard, E.C.Aksoy, S.Hide, W.[Response to Hertz-Fowler and Berriman: Continuing tsetse and trypanosoma genome sequencing projects]Response to Hertz-Fowler and Berriman: Continuing tsetse and trypanosoma genome sequencing projects

Acute Trypanosoma congolense infection induced moderate, transient anemia in N'Dama cattle (trypanotolerant) and severe anemia in Boran cattle (trypanosusceptible). Erythropoietin receptor (EpoR) was cloned and sequenced from the two breeds of cattle. A single position mutation of Tyr in the Bora...


Suliman, H.B.Logan-Henfrey, L.L.Majiwa, P.A.O.Ole-MoiYoi, O.K.Feldman, B.F.[Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense]Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense

A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...


Masake, R.A.Ole-MoiYoi, O.K.Urakawa, T.Hirumi, H.Majiwa, P.A.O.Wells, C.W.Minja, S.H.Makau, J.M.Nantulya, V.M.[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

The complete nucleotide sequences were determined for three transcripts each encoding a different variant surface glycoprotein (VSG) of Trypanosoma (Nannomonas) congolense. The nucleotide sequence was determined also for a transcript encoding a fourth VSG, but this was truncated. The data obtaine...


Urakawa, T.Eshita, Y.Majiwa, P.A.O.[The primary structure of Trypanosoma (Nannomonas) congolense variant surface glycoproteins]The primary structure of Trypanosoma (Nannomonas) congolense variant surface glycoproteins

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