A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T. evansi infection in livestock, was cloned as a recombinant plasmid and sequenced. A recombinant baculov...
Urakawa, T.
,
Verloo, D.
,
Moens, L.
,
Buscher, P.
,
Majiwa, P.A.O.
,
[Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2]
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Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2
Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then ...
Majiwa, P.A.O.
,
Hamers, R.
,
Meirvenne, N. van
,
Matthyssens, G.
,
[Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense]
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Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense
Knowles, G.
,
Betschart, K.
,
Kukla, B.A.
,
Scott, J.R.
,
Majiwa, P.A.O.
,
[Genetically discrete populations of Trypanosoma congolense from livestock on the Kenyan coast]
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Genetically discrete populations of Trypanosoma congolense from livestock on the Kenyan coast
The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. All T. evansi isolates hitherto tested express this VAT. In a study on the differential diagnosis of T. equiperdum and T. evansi in horses, we investigated serolo...
Claes, F.
,
Verloo, D.
,
Waal, D.T. de
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Urakawa, T.
,
Majiwa, P.A.O.
,
Goddeeris, B.M.
,
Buscher, P.
,
[Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum]
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Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum
Tsetse flies Glossina spp. (Diptera: Glossinidae) harbor three different symbiotic microorganisms one being an intracellular Rickettsia of the genus Wolbachia. This bacterium infects a wide range of arthropods, where it causes a variety of reproductive abnormalities, one of which is termed cytopl...
Cheng, Q
,
Ruel, T.D.
,
Zhou, W.
,
Moloo, S.K.
,
Majiwa, P.A.O.
,
O'Neill, S.L.
,
Aksoy, S.
,
[Tissue distribution and prevalence of Wolbachia infections in tsetse flies, Glossina spp.]
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Tissue distribution and prevalence of Wolbachia infections in tsetse flies, Glossina spp.
To clarify evolution and phylogenetic relationships of trypanosome alternative oxidase (AOX) molecules, AOX genes (cDNAs) of the African trypanosomes, Trypanosoma congolense and Trypanosoma evansi, were cloned by PCR. Both AOXs possess conserved consensus motifs (-E-, -EXXH-). The putative amino ...
Suzuki, T.
,
Hashimoto, T.
,
Yabu, Y.
,
Majiwa, P.A.O.
,
Ohshima, S.
,
Suzuki, M.
,
Lu, S.
,
Hato, M.
,
Kido, Y.
,
Sakamoto, K.
,
Nakamura, K.
,
Kita, K.
,
Ohta, N.
,
[Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families]
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Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families