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Trypanosomosis remains one of the biggest impediments to the Development of the livestock industry in sub-Saharan Africa while it is likely that a vaccine for this disease could be found in the future, none is presently available. Mean-while different strategies are being followed to control this...


Majiwa, P.A.O.Konde, V.Souza, M. dePeregrine, A.S.Maina, M.[Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense]Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense

Chemotherapy is the single most important strategy for the control of trypanosomosis in African livestock. Trypanocidal drug resistance is a growing problem across much of sub-Saharan Africa and increasingly recognised as a major threat to livestock production. It is therefore of pressing importa...


Coleman, P.G.Eisler, M.C.McDermott, John J.Murphy, N.B.Majiwa, P.A.O.Peregrine, A.S.[Relative fitness in laboratory mice of drug resistant and drug-sensitive field isolates of Trypanosoma congolense]Relative fitness in laboratory mice of drug resistant and drug-sensitive field isolates of Trypanosoma congolense

In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic...


Konde, V.Majiwa, P.A.O.[Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense]Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense

Studies on infections with drug-resistant trypanosomes indicate that the majority of parasites in a population are sensitive to the drug dose used to select the population (Mamman et al., 1993). This study was initiated to examine whether a drug-resistant trypanosome population could influence th...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Murphy, N.B.[Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction]Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction

Two clones of Trypanosoma (Nannomonas) congolense were derived one from the other by growth in mice and administration of increasing sub-curative doses of isometamidium chloride. One of the clones thus became approximately 10x more tolerant to the trypanocide than the other mRNA from the two clon...


Majiwa, P.A.O.Maina, M.Peregrine, A.S.Kasumba, I.[Comparative molecular analysis of isogenic clones of Trypanosoma (Nanommonas) congolense that differ in their response to isometamidium chloride]Comparative molecular analysis of isogenic clones of Trypanosoma (Nanommonas) congolense that differ in their response to isometamidium chloride

Tsetse flies Glossina spp. (Diptera: Glossinidae) harbor three different symbiotic microorganisms one being an intracellular Rickettsia of the genus Wolbachia. This bacterium infects a wide range of arthropods, where it causes a variety of reproductive abnormalities, one of which is termed cytopl...


Cheng, QRuel, T.D.Zhou, W.Moloo, S.K.Majiwa, P.A.O.O'Neill, S.L.Aksoy, S.[Tissue distribution and prevalence of Wolbachia infections in tsetse flies, Glossina spp.]Tissue distribution and prevalence of Wolbachia infections in tsetse flies, Glossina spp.

To clarify evolution and phylogenetic relationships of trypanosome alternative oxidase (AOX) molecules, AOX genes (cDNAs) of the African trypanosomes, Trypanosoma congolense and Trypanosoma evansi, were cloned by PCR. Both AOXs possess conserved consensus motifs (-E-, -EXXH-). The putative amino ...


Suzuki, T.Hashimoto, T.Yabu, Y.Majiwa, P.A.O.Ohshima, S.Suzuki, M.Lu, S.Hato, M.Kido, Y.Sakamoto, K.Nakamura, K.Kita, K.Ohta, N.[Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families]Alternative Oxidase (AOX) Genes of African Trypanosomes: Phylogeny and Evolution of AOX and Plastid Terminal Oxidase families

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