In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T...
Claes, F.
,
Verloo, D.
,
Waal, D.T. de
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Majiwa, P.A.O.
,
Baltz, T.
,
Goddeeris, B.M.
,
Buscher, P.
,
[The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum]
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The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum
The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary ca...
Lejon, Veerle
,
Claes, F.
,
Verloo, D.
,
Maina, M.
,
Urakawa, T.
,
Majiwa, P.A.O.
,
Buscher, P.
,
[Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels]
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Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
,
Ole-MoiYoi, O.K.
,
Urakawa, T.
,
Hirumi, H.
,
Majiwa, P.A.O.
,
Wells, C.W.
,
Minja, S.H.
,
Makau, J.M.
,
Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals