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Williams, R.O.Young, J.R.Majiwa, P.A.O.Doyle, J.J.Shapiro, S.Z.[Analyses of variable antigen gene rearrangements in Trypanosoma brucei]Analyses of variable antigen gene rearrangements in Trypanosoma brucei

In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T...


Claes, F.Verloo, D.Waal, D.T. deMajiwa, P.A.O.Baltz, T.Goddeeris, B.M.Buscher, P.[The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum]The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum
Williams, R.O.Young, J.R.Majiwa, P.A.O.[Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei]Genomic rearrangements correlated with antigenic variation in Trypanosoma brucei
Young, J.R.Donelson, J.E.Majiwa, P.A.O.Shapiro, S.Z.Williams, R.O.[Analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei]Analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei
Majiwa, P.A.O.Young, J.R.Englund, P.T.Shapiro, S.Z.Williams, R.O.[Two distinct forms of surface antigen gene rearrangements in Trypanosoma brucei]Two distinct forms of surface antigen gene rearrangements in Trypanosoma brucei

Transcripts which encode two metacyclic-form-specific variable surface glycoproteins (mVSGs) of Trypanosoma congolense IL3000 have been cloned into baculovirus expression vectors using a novel transfer vector, pAcL11. One of the recombinant baculoviruses (AcVSG1) expressed a mVSG as a glycoprotei...


Urakawa, T.Eshita, Y.Fukuma, T.Hirumi, H.Hirumi, K.Majiwa, P.A.O.[Expression of Trypanosoma congolense antigens in spodoptera frugiperdia insect cells]Expression of Trypanosoma congolense antigens in spodoptera frugiperdia insect cells

A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...


Masake, R.A.Ole-MoiYoi, O.K.Urakawa, T.Hirumi, H.Majiwa, P.A.O.Wells, C.W.Minja, S.H.Makau, J.M.Nantulya, V.M.[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T. evansi infection in livestock, was cloned as a recombinant plasmid and sequenced. A recombinant baculov...


Urakawa, T.Verloo, D.Moens, L.Buscher, P.Majiwa, P.A.O.[Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2]Trypanosoma evansi: Cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2
Williams, R.O.Young, J.R.Majiwa, P.A.O.Doyle, J.J.Shapiro, S.Z.[Contextural genomic rearrangements of variable-antigen genes in Trypanosoma brucei]Contextural genomic rearrangements of variable-antigen genes in Trypanosoma brucei

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