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Previous molecular genetic studies on the African trypanosome have focused on only a few genes and gene products, the majority of which are concerned with surface antigenic variation: consequently, an insignificant number of the genes of this organism have been characterized to date. In order to:...


Djikeng, AppolinaireAgufa, C.Donelson, J.E.Majiwa, P.A.O.[Generation of expressed sequence tags as physical landmarks in the genome of Trypanosoma brucei]Generation of expressed sequence tags as physical landmarks in the genome of Trypanosoma brucei
Donelson, J.E.Majiwa, P.A.O.Williams, R.O.[Kinetoplast DNA minicircles of Trypanosoma brucei share regions of sequence homology]Kinetoplast DNA minicircles of Trypanosoma brucei share regions of sequence homology
Majiwa, P.A.O.Otieno, L.H.[Recombinant DNA probes reveal simultaneous infection of tsetse flies with different trypanosome species]Recombinant DNA probes reveal simultaneous infection of tsetse flies with different trypanosome species
Kukla, B.A.Majiwa, P.A.O.Young, J.R.Moloo, S.K.Ole-MoiYoi, O.K.[Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies]Use of species-specific DNA probes for detection and identification of trypanosome infection in tsetse flies

Isometamidium chloride has remained a very important prophylactic and therapeutic drug against trypanosomosis in cattle since its introduction into the market in the 1950s with, unfortunately, a concomitant development of resistance in trypanosomosis endemic areas. Amplified Fragment Length Polym...


Delespaux, V.Geysen, D.Majiwa, P.A.O.Geerts, S.[Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense]Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense

During its first two years, the African trypanosome genome project, funded in part by the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), has concentrated on three fundamental areas: (1) gene discovery by single-pass sequencing of cDNAs to produce expre...


Melville, S.E.Majiwa, P.A.O.Donelson, J.E.[Resources available from the African Trypanosome Genome Project]Resources available from the African Trypanosome Genome Project

Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation i...


Urakawa, T.Majiwa, P.A.O.[Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense]Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense

Polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) probes were used to characterize trypanosomes from cattle in Morogoro region of Tanzania. Blood samples collected from 390 beef and dairy cattle in selected farms in Morogoro region were examined for presence of trypanosomes using th...


Mugittu, K.N.Silayo, R.S.Majiwa, P.A.O.Kimbita, E.K.Mutayoba, B.M.MaSelle, R.[Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania]Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania

A study was carried out to determine whether a drug-resistant trypanosome population could influence the survival of a drug-sensitive population in mixed infections in goats. To identify both populations during the course of a mixed infection, a system for distinguishing them was developed; using...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Mbiuki, S.M.Murphy, N.B.[Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats]Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats

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