The pathogenic trypanosomes Trypanosoma vivax and Trypanosoma congolense are transmitted by tsetse and cause trypanosomosis, a debilitating disease affecting livestock in Africa and South America. Here, we discuss the need, relevance and advantage of having the Trypanosoma genomes sequenced, an...
Davila, A.M.R.
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Majiwa, P.A.O.
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Grisard, E.C.
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Aksoy, S.
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Melville, S.E.
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[Comparative genomics to uncover the secrets of tsetse and livestock-infective trypanosomes]
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Comparative genomics to uncover the secrets of tsetse and livestock-infective trypanosomes
Polymerase chain reaction (PCR) and deoxyribonucleic acid (DNA) probes were used to characterize trypanosomes from cattle in Morogoro region of Tanzania. Blood samples collected from 390 beef and dairy cattle in selected farms in Morogoro region were examined for presence of trypanosomes using th...
Mugittu, K.N.
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Silayo, R.S.
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Majiwa, P.A.O.
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Kimbita, E.K.
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Mutayoba, B.M.
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MaSelle, R.
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[Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania]
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Application of PCR and DNA probes in the characterisation of trypanosomes in the blood of cattle in farms in Morogoro, Tanzania
Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...
Masake, R.A.
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Njuguna, J.T.
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Brown, C.C.
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Majiwa, P.A.O.
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[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]
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The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock
The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...
Masake, R.A.
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Majiwa, P.A.O.
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Moloo, S.K.
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Makau, J.M.
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Njuguna, J.
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Maina, M.
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Kabata, J.M.
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Ole-MoiYoi, O.K.
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Nantulya, V.M.
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[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]
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Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction
The recent genome initiatives of the neglected parasites Trypanosoma vivax and Trypanosoma congolense, and the vector tsetse are promising with regards to the data available in public databases. While these genome initiatives and the potential for developing new drugs and vaccines are admirable, ...
Davila, A.M.R.
,
Majiwa, P.A.O.
,
Grisard, E.C.
,
Aksoy, S.
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Hide, W.
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[Response to Hertz-Fowler and Berriman: Continuing tsetse and trypanosoma genome sequencing projects]
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Response to Hertz-Fowler and Berriman: Continuing tsetse and trypanosoma genome sequencing projects
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
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Ole-MoiYoi, O.K.
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Urakawa, T.
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Hirumi, H.
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Majiwa, P.A.O.
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Wells, C.W.
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Minja, S.H.
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Makau, J.M.
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Nantulya, V.M.
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[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
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Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals