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Accurate identification of an infectious organism facilitates the study of epidemiology of the disease it causes and makes possible rational evaluation of the relative success of different control strategies. Highly repetitive, tandem or interspersed DNA sequences unique to different species, typ...


Majiwa, P.A.O.[DNA probe- and PCR-based methods for the detection of trypanosomes]DNA probe- and PCR-based methods for the detection of trypanosomes

Drug resistance in trypanosomes is increasingly being recognised as a constraint to livestock production in many parts of Africa. However the long-term stability of such resistance is unclear and has therefore been investigated over a 4-year period at a site in Ethiopia. The result shows that the...


Peregrine, A.S.Majiwa, P.A.O.Rowlands, G.J.[Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia]Long-term occurrence of multiple drug resistance in Trypanosoma congolense at Ghibe, Ethiopia

Trypanosomosis remains one of the biggest impediments to the Development of the livestock industry in sub-Saharan Africa while it is likely that a vaccine for this disease could be found in the future, none is presently available. Mean-while different strategies are being followed to control this...


Majiwa, P.A.O.Konde, V.Souza, M. dePeregrine, A.S.Maina, M.[Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense]Molecular approaches to the identification of DNA markers for drug resistance in Trypanosoma congolense

Chemotherapy is the single most important strategy for the control of trypanosomosis in African livestock. Trypanocidal drug resistance is a growing problem across much of sub-Saharan Africa and increasingly recognised as a major threat to livestock production. It is therefore of pressing importa...


Coleman, P.G.Eisler, M.C.McDermott, John J.Murphy, N.B.Majiwa, P.A.O.Peregrine, A.S.[Relative fitness in laboratory mice of drug resistant and drug-sensitive field isolates of Trypanosoma congolense]Relative fitness in laboratory mice of drug resistant and drug-sensitive field isolates of Trypanosoma congolense

In order to search for T. congolense genes whose transcription status may be influenced by response to isometamidium chloride, we applied the Representational Difference Analysis (RDA) (Lisitsyn et al., 1993; Hubank & Schatz, 1994) to the comparative analysis of cDNA synthesised from two isogenic...


Konde, V.Majiwa, P.A.O.[Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense]Alterations in gene transcription patterns associated with the Development of isometamidium resistance in Trypanosoma congolense

Studies on infections with drug-resistant trypanosomes indicate that the majority of parasites in a population are sensitive to the drug dose used to select the population (Mamman et al., 1993). This study was initiated to examine whether a drug-resistant trypanosome population could influence th...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Murphy, N.B.[Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction]Evaluation of response to treatment of mixed trypanosome infection in goats using the polymerase chain reaction

Two clones of Trypanosoma (Nannomonas) congolense were derived one from the other by growth in mice and administration of increasing sub-curative doses of isometamidium chloride. One of the clones thus became approximately 10x more tolerant to the trypanocide than the other mRNA from the two clon...


Majiwa, P.A.O.Maina, M.Peregrine, A.S.Kasumba, I.[Comparative molecular analysis of isogenic clones of Trypanosoma (Nanommonas) congolense that differ in their response to isometamidium chloride]Comparative molecular analysis of isogenic clones of Trypanosoma (Nanommonas) congolense that differ in their response to isometamidium chloride

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