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Maruthi, M.N.Whitfield, E.C.Kumar, P.Legg, J.P.[Virusindexing, chemo and thermo therapies, and micro propagation for generating virusfree cassava plants: a labouratory manual]Virusindexing, chemo and thermo therapies, and micro propagation for generating virusfree cassava plants: a labouratory manual

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringen...


Anjanappa, R.B.Mehta, D.Maruthi, M.N.Kanju, E.Gruissem, W.Vanderschuren, H.[Characterization of brown streak virus–resistant cassava]Characterization of brown streak virus–resistant cassava

Tumwegamire, SilverKanju, E.Legg, J.P.Shirima, R.Kombo, S.Mkamilo, G.S.Mtunda, K.Sichalwe, K.Kulembeka, H.P.Ndyetabura, I.Saleh, H.Kawuki, R.Alicai, TitusAdiga, G.Benesi, I.Mhone, A.Zacarias, A.Matsimbe, S.F.Munga, T.Ateka, E.Navangi, L.Maruthi, M.N.Mwatuni, F.Ngundo, G.Mwangangi, M.Mbugua, E.Ndunguru, J.Rajabu, C.Mark, D.[Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa]Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa

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