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Aomongo, Christopher A.Kawuki, Robert S.Bellotti, Anthony C.Alicai, TitusBaguma, Yona K.Maruthi, M.N.Bua, AntonColvin, John[African cassava whitefly, Bemisia tabaci, resistance in African and South American cassava genotypes]African cassava whitefly, Bemisia tabaci, resistance in African and South American cassava genotypes

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...


Abarshi, M.M.Mohammed, I.Jeremiah, S.C.Legg, J.P.Kumar, P.L.Hillocks, R.J.Maruthi, M.N.[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringen...


Anjanappa, R.B.Mehta, D.Maruthi, M.N.Kanju, E.Gruissem, W.Vanderschuren, H.[Characterization of brown streak virus–resistant cassava]Characterization of brown streak virus–resistant cassava

Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture...


Maruthi, M.N.Whitfield, E.C.Otti, G.Tumwegamire, SilverKanju, E.Legg, J.P.Mkamilo, G.Kawuki, R.Benesi, I.Zacarias, A.Munga, T.Mwatuni, F.Mbugua, E.[A method for generating virus-free cassava plants to combat viral disease epidemics in Africa]A method for generating virus-free cassava plants to combat viral disease epidemics in Africa

Tumwegamire, SilverKanju, E.Legg, J.P.Shirima, R.Kombo, S.Mkamilo, G.S.Mtunda, K.Sichalwe, K.Kulembeka, H.P.Ndyetabura, I.Saleh, H.Kawuki, R.Alicai, TitusAdiga, G.Benesi, I.Mhone, A.Zacarias, A.Matsimbe, S.F.Munga, T.Ateka, E.Navangi, L.Maruthi, M.N.Mwatuni, F.Ngundo, G.Mwangangi, M.Mbugua, E.Ndunguru, J.Rajabu, C.Mark, D.[Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa]Exchanging and managing in-vitro elite germplasm to combat cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) in Eastern and Southern Africa

Cassava brown streak disease (CBSD) is arguably the most dangerous current threat to cassava, which is Africa's most important food security crop. CBSD is caused by two RNA viruses: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). The roles of the whitefly Bemisia...


Maruthi, M.N.Jeremiah, S.C.Mohammed, I.U.Legg, J.P.[The role of the whitefly, Bemisia tabaci (Gennadius), and farmer practices in the spread of cassava brown streak ipomoviruses]The role of the whitefly, Bemisia tabaci (Gennadius), and farmer practices in the spread of cassava brown streak ipomoviruses
Patil, B.L.Ogwok, E.Wagaba, HenryMohammed, I.U.Yadav, J.S.Bagewadi, B.Taylor, Nigel J.Kreuze, J.F.Maruthi, M.N.Alicai, TitusFauquet, Claude M.[RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease.]RNAi-mediated resistance to diverse isolates belonging to two virus species involved in Cassava brown streak disease.

In April 2008 in the northern Provinces of Angola, banana (AAA genome)and plantain (AAB genome) plants (Musa spp.) with severe stunting andmoderate to severe leaf symptoms typical of banana bunchy top diseasewere observed to be widespread in farmers’ fields in Kirimadhola villagein the Ndalatando...


Kumar, P.L.Ayodele, M.Oben, T.T.Mahungu, N.M.Beed, Fen D.Coyne, D.L.Londa, L.Mutunda, M.P.Kiala, D.Maruthi, M.N.[First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola]First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola

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