Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...
Abarshi, M.M.
,
Mohammed, I.
,
Jeremiah, S.C.
,
Legg, J.P.
,
Kumar, P.L.
,
Hillocks, R.J.
,
Maruthi, M.N.
,
[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]
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Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa
Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa.
Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...
Otti, G.
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Bouvaine, S.
,
Kimata, B.
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Mkamillo, G.
,
Kumar, P.L.
,
Tomlins, Keith I.
,
Maruthi, M.N.
,
[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]
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High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants
In April 2008 in the northern Provinces of Angola, banana (AAA genome)and plantain (AAB genome) plants (Musa spp.) with severe stunting andmoderate to severe leaf symptoms typical of banana bunchy top diseasewere observed to be widespread in farmers’ fields in Kirimadhola villagein the Ndalatando...
Kumar, P.L.
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Ayodele, M.
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Oben, T.T.
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Mahungu, N.M.
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Beed, Fen D.
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Coyne, D.L.
,
Londa, L.
,
Mutunda, M.P.
,
Kiala, D.
,
Maruthi, M.N.
,
[First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola]
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First report of banana bunchy top virus in banana and plantain (Musa spp.) in Angola