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Journal Article 2000

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Njuguna, J.T. Ndung'u, J.M.

A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...

Enlace original: https://cgspace.cgiar.org/handle/10568/29977

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

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Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Njuguna, J.T. , Ndung'u, J.M. , [Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)] , Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Journal Article 1998

Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

Autor: Eisler, M.C. Lessard, P. Masake, R.A. Moloo, S.K. Peregrine, A.S.

Sensitivity and specificity of the FAO/IAEA kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (three populations) or T. vivax (one population). The kits are based on mono...

Enlace original: https://cgspace.cgiar.org/handle/10568/33243

Investigación en Agricultura

CATTLE

TRYPANOSOMA CONGOLENSE

TRYPANOSOMA VIVAX

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Eisler, M.C. , Lessard, P. , Masake, R.A. , Moloo, S.K. , Peregrine, A.S. , [Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle] , Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

Journal Article 2002

The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock

Autor: Masake, R.A. Njuguna, J.T. Brown, C.C. Majiwa, P.A.O.

Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...

Enlace original: https://cgspace.cgiar.org/handle/10568/33256

Investigación en Agricultura

TRYPANOSOMA BRUCEI

TRYPANOSOMA VIVAX

PCR

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Masake, R.A. , Njuguna, J.T. , Brown, C.C. , Majiwa, P.A.O. , [The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock] , The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock

Journal Article 1996

Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Autor: Bosompem, K.M. Masake, R.A. Assoku, R.K.G. Opiyo, E.A. Nantulya, V.M.

A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected wi...

Enlace original: https://cgspace.cgiar.org/handle/10568/29386

Investigación en Agricultura

TRYPANOSOMES (PHYTOMONAS)

GLOSSINIDAE

EVALUATION

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Bosompem, K.M. , Masake, R.A. , Assoku, R.K.G. , Opiyo, E.A. , Nantulya, V.M. , [Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)] , Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Journal Article 1997

Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Autor: Masake, R.A. Majiwa, P.A.O. Moloo, S.K. Makau, J.M. Njuguna, J. Maina, M. Kabata, J.M. Ole-MoiYoi, O.K. Nantulya, V.M.

The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...

Enlace original: https://cgspace.cgiar.org/handle/10568/28613

Investigación en Agricultura

TRYPANOSOMA VIVAX

DNA

ELISA

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Masake, R.A. , Majiwa, P.A.O. , Moloo, S.K. , Makau, J.M. , Njuguna, J. , Maina, M. , Kabata, J.M. , Ole-MoiYoi, O.K. , Nantulya, V.M. , [Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction] , Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Conference Paper 1995

Diagnosis of African trypanosomosis

Autor: Masake, R.A.

Diagnosis is an essential requirement in the management of disease both at the level of the individual, when a decision has to be made whether to treat or not, and at the epidemiological level for evaluating the performance of disease control strategies. The centrality of diagnosis in disease man...

Enlace original: https://cgspace.cgiar.org/handle/10568/50385

Investigación en Agricultura

TRYPANOSOMIASIS

DIAGNOSIS

ELISA

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Masake, R.A. , [Diagnosis of African trypanosomosis] , Diagnosis of African trypanosomosis

Conference Paper 1999

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Minja, S.H. Njuguna, J.T. Olaho-Mukani, W. Ndung'u, J.M.

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...

Enlace original: https://cgspace.cgiar.org/handle/10568/50294

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

Citar Enlace permanente

Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Minja, S.H. , Njuguna, J.T. , Olaho-Mukani, W. , Ndung'u, J.M. , [Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)] , Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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Resultados de búsqueda

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock

Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Diagnosis of African trypanosomosis

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)