Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...
Masake, R.A.
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Njuguna, J.T.
,
Brown, C.C.
,
Majiwa, P.A.O.
,
[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]
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The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock
The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...
Masake, R.A.
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Majiwa, P.A.O.
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Moloo, S.K.
,
Makau, J.M.
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Njuguna, J.
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Maina, M.
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Kabata, J.M.
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Ole-MoiYoi, O.K.
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Nantulya, V.M.
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[Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction]
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Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction
Diagnosis is an essential requirement in the management of disease both at the level of the individual, when a decision has to be made whether to treat or not, and at the epidemiological level for evaluating the performance of disease control strategies. The centrality of diagnosis in disease man...
Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...
Ouma, J.O.
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Masake, R.A.
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Masiga, Daniel K.
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Moloo, S.K.
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Minja, S.H.
,
Njuguna, J.T.
,
Olaho-Mukani, W.
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Ndung'u, J.M.
,
[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]
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Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)