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The FAO/IAEA Trypanosomiasis Direct Antigen-ELISA kit has been distributed to scientists holding research contracts under an IAEA Co-ordinated Research Programme in 14 sub-Saharan African countries. It is intended for improved diagnosis of bovine tsetse-transmitted trypanosomiasis as an aid to e...


Eisler, M.C.Lessard, P.Moloo, D.Masake, R.A.Peregrine, A.S.[Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypanosoma vivax infections in cattle]Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypanosoma vivax infections in cattle

Specific humoral and cell mediated immunity to blood parasites is reviewed. There is a table detailing the worldwide occurrence of economically important blood parasitic diseases followed by summaries of immunological aspects of trypanosomosis, theileriosis, babesiosis, anaplasmosis, cowdriosis a...


Musoke, A.J.Masake, R.A.[Blood parasitic diseases and specific immune responses]Blood parasitic diseases and specific immune responses

Advances in biotechnological techniques are driven by the needs of the human populations. The new techniques in biotechnology have been developed as a consequence of the integrated use of microbiology, biochemistry and chemical engineering. Some of these technologies are being employed in the der...


Masake, R.A.Musoke, A.J.Ole-MoiYoi, O.K.[Application of biotechnology in disease diagnosis and vaccine Development]Application of biotechnology in disease diagnosis and vaccine Development
Autor: Masake, R.A.

Diagnosis is an essential requirement in the management of disease both at the level of the individual, when a decision has to be made whether to treat or not, and at the epidemiological level for evaluating the performance of disease control strategies. The centrality of diagnosis in disease man...


Masake, R.A.[Diagnosis of African trypanosomosis]Diagnosis of African trypanosomosis

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...


Ouma, J.O.Masake, R.A.Masiga, Daniel K.Moloo, S.K.Minja, S.H.Njuguna, J.T.Olaho-Mukani, W.Ndung'u, J.M.[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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