Four Boran cattle were infected with Trypanosoma brucei using Glossina morsitans centralis and were left untreated throughout the experimental period of 18 months. During this period, sequential blood samples were collected and examined for the presence of anti-trypanosome antibodies and their an...
Masake, R.A.
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Moloo, S.K.
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Nantulya, V.M.
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Minja, S.H.
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Makau, J.M.
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Njuguna, J.T.
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[Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle]
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Comparative sensitivity of antigen-detection enzyme immunosorbent assay and the microhaematocrit centrifugation technique in the diagnosis of Trypanosoma brucei infections in cattle
A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...
Ouma, J.O.
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Masake, R.A.
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Masiga, Daniel K.
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Moloo, S.K.
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Njuguna, J.T.
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Ndung'u, J.M.
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[Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)]
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Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)
Susceptibility of African buffalo, eland, waterbuck, N'Dama and Boran cattle to sequential Glossina morsitans centralis-transmitted infections of Trypanosoma congolense, T. brucei brucei and T. vivax was compared, and their possible role as reservoirs of these parasites for G. moristans centralis...
Moloo, S.K.
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Orinda, G.O.
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Sabwa, C.L.
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Minja, S.H.
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Masake, R.A.
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[Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle]
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Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle
Following an outbreak of trypanosomosis in horses on a farm in Kenya, 18 trypanosome isolates were collected from the infected animals over a period of one and a half years and cryopreserved for characterization. The characterization was done on the basis of morphology using Giemsa-stained blood ...
Kihurani, D.O.
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Masake, R.A.
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Nantulya, V.M.
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Mbiuki, S.M.
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[Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya]
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Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya
Teneral tsetse flies infected with either Trvpanosoma brucei or T. vivax were fed on healthy cattle. Blood samples collected daily from the cattle were examined by microscopy for the presence of trypanosomes, in thick smear, thin smear and in the buffy coat (BC). All the cattle fed upon by infect...
Masake, R.A.
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Njuguna, J.T.
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Brown, C.C.
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Majiwa, P.A.O.
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[The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock]
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The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock