A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Njuguna, J.T.
,
Ndung'u, J.M.
,
[Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)]
,
Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)
Studies the pathology of Trypanosoma vivax in East African goats and Friesian cattle, with particular. ref. to goat & cattle parasitemia, susceptibility of goats over cattle in terms of higher parasitaemias, severe anaemia & death; describes major pathological changes occurring in lymph nodes, sp...
Masake, R.A.
,
[The pathogenesis of infection with Trypanosoma vivax in goats and cattle]
,
The pathogenesis of infection with Trypanosoma vivax in goats and cattle
A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...
Masake, R.A.
,
Ole-MoiYoi, O.K.
,
Urakawa, T.
,
Hirumi, H.
,
Majiwa, P.A.O.
,
Wells, C.W.
,
Minja, S.H.
,
Makau, J.M.
,
Nantulya, V.M.
,
[Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals]
,
Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals
Specific humoral and cell mediated immunity to blood parasites is reviewed. There is a table detailing the worldwide occurrence of economically important blood parasitic diseases followed by summaries of immunological aspects of trypanosomosis, theileriosis, babesiosis, anaplasmosis, cowdriosis a...
Diagnosis is an essential requirement in the management of disease both at the level of the individual, when a decision has to be made whether to treat or not, and at the epidemiological level for evaluating the performance of disease control strategies. The centrality of diagnosis in disease man...
Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Minja, S.H.
,
Njuguna, J.T.
,
Olaho-Mukani, W.
,
Ndung'u, J.M.
,
[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]
,
Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)