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Journal Article 2000

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Njuguna, J.T. Ndung'u, J.M.

A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...

Enlace original: https://cgspace.cgiar.org/handle/10568/29977

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

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Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Njuguna, J.T. , Ndung'u, J.M. , [Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)] , Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Journal Article 1999

Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle

Autor: Moloo, S.K. Orinda, G.O. Sabwa, C.L. Minja, S.H. Masake, R.A.

Susceptibility of African buffalo, eland, waterbuck, N'Dama and Boran cattle to sequential Glossina morsitans centralis-transmitted infections of Trypanosoma congolense, T. brucei brucei and T. vivax was compared, and their possible role as reservoirs of these parasites for G. moristans centralis...

Enlace original: https://cgspace.cgiar.org/handle/10568/33246

Investigación en Agricultura

TRYPANOSOMA CONGOLENSE

TRYPANOSOMA BRUCEI

CATTLE

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Moloo, S.K. , Orinda, G.O. , Sabwa, C.L. , Minja, S.H. , Masake, R.A. , [Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle] , Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle

Journal Article 1996

Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Autor: Bosompem, K.M. Masake, R.A. Assoku, R.K.G. Opiyo, E.A. Nantulya, V.M.

A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected wi...

Enlace original: https://cgspace.cgiar.org/handle/10568/29386

Investigación en Agricultura

TRYPANOSOMES (PHYTOMONAS)

GLOSSINIDAE

EVALUATION

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Bosompem, K.M. , Masake, R.A. , Assoku, R.K.G. , Opiyo, E.A. , Nantulya, V.M. , [Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)] , Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Journal Article 1995

Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Autor: Masake, R.A. Ole-MoiYoi, O.K. Urakawa, T. Hirumi, H. Majiwa, P.A.O. Wells, C.W. Minja, S.H. Makau, J.M. Nantulya, V.M.

A monoclonal antibody (MAb)Tv27 employed in an antigen-detection enzyme immunosorbent assay (Ag-ELISA) for diagnosis of Trypanosoma vivax infection was shown to react with a T. vivax-specific protein of an approximate molecular weight of 10 kDa. This protein is diffusely distributed throughout th...

Enlace original: https://cgspace.cgiar.org/handle/10568/28608

Investigación en Agricultura

TRYPANOSOMIASIS

TRYPANOSOMA VIVAX

ESCHERICHIA COLI

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Masake, R.A. , Ole-MoiYoi, O.K. , Urakawa, T. , Hirumi, H. , Majiwa, P.A.O. , Wells, C.W. , Minja, S.H. , Makau, J.M. , Nantulya, V.M. , [Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals] , Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Conference Paper 1999

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Minja, S.H. Njuguna, J.T. Olaho-Mukani, W. Ndung'u, J.M.

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...

Enlace original: https://cgspace.cgiar.org/handle/10568/50294

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

Citar Enlace permanente

Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Minja, S.H. , Njuguna, J.T. , Olaho-Mukani, W. , Ndung'u, J.M. , [Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)] , Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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Resultados de búsqueda

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Study on the sequential tsetse-transmitted Trypanosoma congolense, T. brucei brucei and T. vivax infections to African buffalo, eland, waterbuck, N'Dama and Boran cattle

Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossina spp.)

Immunological characterization and expression in Escherichia coli and Baculovirus systems of a Trypanosoma vivax antigen detected in the blood of infected animals

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)