Napier grass Stunt Disease (NSD) is a severe disease of Napier grass (Pennisetum purpureum) in Eastern Africa, caused by the leafhopper-transmitted bacterium Candidatus Phytoplasma oryzae. The pathogen severely impairs the growth of Napier grass, the major fodder for dairy cattle in Eastern Afric...
Wambua, L.
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Schneider, B.
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Okwaro, A.
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Wanga, J.O.
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Imali, O.
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Wambua, P.N.
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Agutu, L.
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Olds, Cassandra L.
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Jones, Christopher S.
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Masiga, Daniel K.
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Midega, C.A.O.
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Khana, Z.
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Jores, Joerg
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Fischer, A.
,
[Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae]
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Development of field-applicable tests for rapid and sensitive detection of Candidatus Phytoplasma oryzae
A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Njuguna, J.T.
,
Ndung'u, J.M.
,
[Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)]
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Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)
Obura, E.
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Midega, C.A.O.
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Khan, Z.R.
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Pickett, J.
,
Masiga, Daniel K.
,
[Napier stunt disease is transmitted by a leafhopper vector Maiestas (=Recilia) banda in Western Kenya]
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Napier stunt disease is transmitted by a leafhopper vector Maiestas (=Recilia) banda in Western Kenya
Gichora, N.N.
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Fatumo, S.A.
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Ngara, M.V.
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Chelbat, N.
,
Ramdayal, K.
,
Villiers, Etienne P. de
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Masiga, Daniel K.
,
Bizzaro, J.W.
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Suravajhala, P.
,
Ommeh, S.C.
,
Hide, W.
,
[Ten simple rules for organizing a virtual conference—anywhere]
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Ten simple rules for organizing a virtual conference—anywhere
An A/G Single Nucleotide Polymorphism (SNP) at position 1,892 of the Mx gene coding sequence has been linked to susceptibility/resistance to avian viral infection in vitro. Using PCR-RFLP and sequencing methods, 1,946 samples from 109 populations from Asia, Africa and Europe; grouped as indigenou...
Ommeh, S.
,
Jin, L.N.
,
Eding, H.
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Muchadeyi, F.C.
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Sulandari, S.
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Danbaro, G.
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Wani, C.E.
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Nie, Q.H.
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Zhang, X. Q.
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Ndila, M.
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Preisinger, R.
,
Chen, G.H.
,
Yousif, I. A.
,
Heo, K.N.
,
Oh, S.J.
,
Tapio, M.
,
Masiga, Daniel K.
,
Hanotte, Olivier H.
,
Weigend, S.
,
Han Jianlin
,
[Geographic and breed distribution patterns of an A/G Polymorphism resent in the Mx gene suggests balanced selection in village chickens]
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Geographic and breed distribution patterns of an A/G Polymorphism resent in the Mx gene suggests balanced selection in village chickens
Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...
Ouma, J.O.
,
Masake, R.A.
,
Masiga, Daniel K.
,
Moloo, S.K.
,
Minja, S.H.
,
Njuguna, J.T.
,
Olaho-Mukani, W.
,
Ndung'u, J.M.
,
[Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)]
,
Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)