Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...
Roa, AC
,
Maya, M.M.
,
Duque E., Myriam Cristina
,
Tohme, Joseph M.
,
Allem, AC
,
Bonierbale, Merideth W.
,
[AFLP analysis of relationships among cassava and other Manihot species]
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AFLP analysis of relationships among cassava and other Manihot species
Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...
Roa, AC
,
Chavarriaga Aguirre, Paul
,
Duque E., Myriam Cristina
,
Maya, M.M.
,
Bonierbale, Merideth W.
,
Iglesias, C.A.
,
Tohme, Joseph M.
,
[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]
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Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship
Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genotyping...
Chavarriaga Aguirre, Paul
,
Maya, M.M.
,
Bonierbale, Merideth W.
,
Kresovich, S
,
Fregene, Martin A.
,
Tohme, Joseph M.
,
Kochert, G
,
[Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability]
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Microsatellites in cassava (Manihot esculenta Crantz) : discovery, inheritance and variability
The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...
Chavarriaga Aguirre, Paul
,
Maya, M.M.
,
Tohme, Joseph M.
,
Duque E., Myriam Cristina
,
Iglesias, C.A.
,
Bonierbale, Merideth W.
,
Kresovich, S
,
Kochert, G
,
[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]
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Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections