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Breeding of Phaseolus vulgaris L. for resistance to common bacterial blight (CBB) can be done with visual evaluations of symptoms to distinguish broad resistance classes, but a more quantitative measure was needed for genetic studies of resistance. A novel method of evaluation was developed by qu...


Constable, ECMichaelis, TEGoodwin, PHMayer, Jorge EdgardPastor Corrales, Marcial A.[Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a breeding program]Evaluation of a DNA probe for the quantitative detection of common bacterial blight in common bean and its application in a breeding program
Hoyos, R.A.Roca, William M.Hosfield, GLMayer, Jorge Edgard[Particle gun transient expression of sexual embryos of common bean]Particle gun transient expression of sexual embryos of common bean
Wenzl, PeterMancilla, Lida I.Rao, Idupulapati M.Mayer, Jorge Edgard[Isolation of rare cDNAs by asymmetric self-hybridization]Isolation of rare cDNAs by asymmetric self-hybridization

Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this...


Chavez, Alba L.Vergara, CEMayer, Jorge Edgard[Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues]Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues

Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...


Ramírez, H.Mayer, Jorge EdgardRoca, William M.Tohme, Joseph M.López, Y.[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético

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