Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this...
Chavez, Alba L.
,
Vergara, CE
,
Mayer, Jorge Edgard
,
[Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues]
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Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues
Aluminum toxicity limits plant growth in acid soils. Because of their advanced state of weathering, acid soils of the tropics also tend to be deficient in nutrients. A realistic assessment of plant adaptation to these soils would therefore require Al-toxic conditions under which growth is simulta...
Wenzl, Peter
,
Mancilla, LI
,
Mayer, Jorge Edgard
,
Albert, R.
,
Rao, Idupulapati M.
,
[Simulating infertile acid soils with nutrient solutions: The effects on Brachiaria species]
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Simulating infertile acid soils with nutrient solutions: The effects on Brachiaria species
Pastor Corrales, Marcial A.
,
Mayer, Jorge Edgard
,
Otoya, María Mercedes
,
Rojas, C
,
[Aplicación de la biología molecular en la fitopatología]
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Aplicación de la biología molecular en la fitopatología
Se construyó una pequeña librería de cDNA de fríjol (phaseolus vulgaris L.) de alrededor de 1500 clones, con el fin de incrementar la saturación del mapa de ligamiento de fríjol con marcadores de RFLPs. Para la generación de la librería se utilizó la técnica de amplificación por PCR (Jepson et al...
Ramírez, H.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
Tohme, Joseph M.
,
López, Y.
,
[Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético]
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Construcción de una genoteca de cDNA de fríjol (Phaseolus vulgaris L.) para mapeo genético
Al resistance of signalgrass (Brachiaria decumbens Stapf cv Basilisk), a widely sown tropical forage grass, is outstanding compared with the closely related ruzigrass (Brachiaria ruziziensis Germain and Evrard cv Common) and Al-resistant genotypes of graminaceous crops such as wheat, triticale, a...
Wenzl, Peter
,
Patino, GM
,
Chavez, Alba L.
,
Mayer, Jorge Edgard
,
Rao, Idupulapati M.
,
[The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices]
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The high level of aluminum resistance in signalgrass is not associated with known mechanism of external aluminum detoxification in root apices
Mayer, Jorge Edgard
,
Niño, R
,
Chuzel, Gérard
,
[Caracterización y determinación de las actividades enzimáticas por técnicas de electroforesis de transferencia y difusión]
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Caracterización y determinación de las actividades enzimáticas por técnicas de electroforesis de transferencia y difusión
Cassava sour starch is a naturally fermented product, which is used in the elaboration of several traditional, flour-based products, like pandeyuca and rosquillas, specially in Colombia and Brazil. Fermentation and drying under the sun confer to the product its specific baking properties (panific...
Flórez, C.
,
Chuzel, Gérard
,
Mayer, Jorge Edgard
,
[Characterization of bacterial amylolytic activities during cassava solid state fermentation]
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Characterization of bacterial amylolytic activities during cassava solid state fermentation
Our strategy for Aqrobacterium-mediated cassava transformation involves the utilization of cotyledonary leaves from somatic embryos and infection with both wild and disarmed strains. Virulence tests for wild Aqrobacterium strains as well as regeneration protocols have been developed; the plasmid ...
Sarria, RA
,
Gómez, A.
,
Cataño C, ML
,
Herrera, P.V.
,
Calderón, A.
,
Mayer, Jorge Edgard
,
Roca, William M.
,
[Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation]
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Towards the development of Agrobacterium tumefaciens and particle bombardment-mediated cassava transformation
Cassava (Manihot esculenta Crantz) is very drought tolerant and hows high levels of photosynthesis at high temperatures. This makes cassava an important source of carbohydrates for several arid regions, especially in Africa. Some varieties perform better than others. Physiological performance, wh...
Tenjo F, FA
,
Mayer, Jorge Edgard
,
[Cloning and sequence analysis of pep-carboxylase from cassava]
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Cloning and sequence analysis of pep-carboxylase from cassava
We want to understand the physiological mechanisms underlying the high photosynthetic rates of cassava under drought stress and high temperatures. Histological analysis of leaf cross sections with cassava PEPC specific antiserum to establish the enzymic compartmentalization pattem is our next goa...
López F, Y.
,
Vélez, W.
,
El-Sharkawy, Mabrouk A.
,
Mayer, Jorge Edgard
,
[Biochemical characterization of PEPC from cassava: a preliminary report]
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Biochemical characterization of PEPC from cassava: a preliminary report