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To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p...


Honda, Y.Waithaka, M.M.Taracha, E.L.N.Duchateau, L.Musoke, A.J.McKeever, Declan J.[Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection]Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection

The protozoan parasite Theileria parva infects and transforms bovine lymphocytes, giving rise to a fatal lympho-proliferative condition known as East Coast fever. Although immune cattle mount strong cytolytic T. lymphocyte responses to the parasite, naive animals appear unable to respond and deve...


McKeever, Declan J.Nyanjui, J.K.Ballingall, K.T.[In vitro infection with Theileria parva is associated with IL10 expression in all bovine lymphocyte lineages]In vitro infection with Theileria parva is associated with IL10 expression in all bovine lymphocyte lineages

There is growing evidence that immunity of cattle to Cowdria ruminantium infection is mediated by T lymphocytes. C. ruminantium antigens that stimulate these responses are therefore of considerable importance to the development of a sub-unit vaccine against the disease. We have examined T cell re...


Mwangi, D.M.McKeever, Declan J.Nyanjui, J.K.Barbet, A.F.Mahan, S.M.[Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent]Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent

Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recentl...


Totte, P.Bensaid, A.Mahan, S.M.Martínez, D.McKeever, Declan J.[Immune responses to Cowdria ruminantium infections]Immune responses to Cowdria ruminantium infections

Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...


Totte, P.Nyanjui, J.Bensaid, A.McKeever, Declan J.[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium

Theileria parva causes an acute lympho-proliferative disease in cattle, which can result in death of susceptible animals within 2-3 weeks of infection. Analyses of the cellular response in the lymph node draining the site of infection demonstrated an early T cell response, with the appearance of ...


Houston, E.F.Taracha, E.L.N.Brackenbury, L.MacHugh, Niall D.McKeever, Declan J.Charleston, B.Morrison, W. Ivan[Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function]Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function

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