To evaluate vaccinia virus as a delivery system for recombinant antigen in cattle, calves were immunized with a recombinant vaccinia virus (rVV) expressing the sporozoite surface antigen (p67) of Theileria parva (V-67) combined with those expressing bovine IL-4 (V-IL4) or IL-2 (V-IL2). The anti-p...
Honda, Y.
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Waithaka, M.M.
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Taracha, E.L.N.
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Duchateau, L.
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Musoke, A.J.
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McKeever, Declan J.
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[Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection]
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Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection
The protozoan parasite Theileria parva infects and transforms bovine lymphocytes, giving rise to a fatal lympho-proliferative condition known as East Coast fever. Although immune cattle mount strong cytolytic T. lymphocyte responses to the parasite, naive animals appear unable to respond and deve...
McKeever, Declan J.
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Nyanjui, J.K.
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Ballingall, K.T.
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[In vitro infection with Theileria parva is associated with IL10 expression in all bovine lymphocyte lineages]
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In vitro infection with Theileria parva is associated with IL10 expression in all bovine lymphocyte lineages
There is growing evidence that immunity of cattle to Cowdria ruminantium infection is mediated by T lymphocytes. C. ruminantium antigens that stimulate these responses are therefore of considerable importance to the development of a sub-unit vaccine against the disease. We have examined T cell re...
Mwangi, D.M.
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McKeever, Declan J.
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Nyanjui, J.K.
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Barbet, A.F.
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Mahan, S.M.
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[Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent]
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Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent
Understanding the basis of protective immunity to Cowdria ruminantium will facilitate the development of an effective subunit vaccine against heartwater in ruminants and contribute to a better definition of protective immune mechanisms to obligate intracellular pathogens in general. Until recentl...
Totte, P.
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Bensaid, A.
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Mahan, S.M.
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Martínez, D.
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McKeever, Declan J.
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[Immune responses to Cowdria ruminantium infections]
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Immune responses to Cowdria ruminantium infections
Cowdria-specific CD4<sup(+)> T-cell lines generated from immunized cattle responded to both soluble and membrane proteins of the agent. The lines produced the Cowdria-inhibitory cytokine IFN-<gamma> in response to soluble antigens fractionated by gel filtration and fast performance liquid chromat...
Totte, P.
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Nyanjui, J.
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Bensaid, A.
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McKeever, Declan J.
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[Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium]
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Bovine CD4<sup(+)> T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium
Theileria parva causes an acute lympho-proliferative disease in cattle, which can result in death of susceptible animals within 2-3 weeks of infection. Analyses of the cellular response in the lymph node draining the site of infection demonstrated an early T cell response, with the appearance of ...
Houston, E.F.
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Taracha, E.L.N.
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Brackenbury, L.
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MacHugh, Niall D.
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McKeever, Declan J.
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Charleston, B.
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Morrison, W. Ivan
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[Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function]
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Infection of cattle with Theileria parva induces an early CD8 T cell response lacking appropriate effector function