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Article

Autor: Figueroa Villalobos, Elías Valdebenito Isler, Iván Merino, O. Ubilla Madrid, Andrea Risopatron, J. Farias, J. G.

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2909

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Merino, O. ,  Ubilla Madrid, Andrea ,  Risopatron, J. ,  Farias, J. G. ,  [Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology] ,  Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
Article

Autor: Risopatron, J. Merino, O. Cheuqueman, C. Figueroa Villalobos, Elías Sanchez, R. Farias, J. G. Valdebenito Isler, Iván

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2944

Repositorio Digital Universidad Católica de Temuco
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Risopatron, J. ,  Merino, O. ,  Cheuqueman, C. ,  Figueroa Villalobos, Elías ,  Sanchez, R. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)] ,  Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Article

Autor: Figueroa Villalobos, Elías Risopatron, J. Sanchez, R. Isachenko, E. Merino, O. Isachenko, V. Valdebenito Isler, Iván

This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3310

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Risopatron, J. ,  Sanchez, R. ,  Isachenko, E. ,  Merino, O. ,  Isachenko, V. ,  Valdebenito Isler, Iván ,  [Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters] ,  Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Article

Autor: Merino, O. Sanchez, R. Risopatron, J. Isachenko, E. Katkov, I. I. Figueroa Villalobos, Elías Valdebenito Isler, Iván Mallmann, P. Isachenko, V.

The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3644

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Sanchez, R. ,  Risopatron, J. ,  Isachenko, E. ,  Katkov, I. I. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Mallmann, P. ,  Isachenko, V. ,  [Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report] ,  Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report
Article

Autor: Merino, O. Risopatron, J. Sanchez, R. Isachenko, E. Figueroa Villalobos, Elías Valdebenito Isler, Iván Isachenko, V.

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3378

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Risopatron, J. ,  Sanchez, R. ,  Isachenko, E. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  [Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness] ,  Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Article

Autor: Magnotti, C. Figueroa Villalobos, Elías Farias, J. G. Merino, O. Valdebenito Isler, Iván Oliveira, R. P. S. Cerqueira, V.

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3840

Repositorio Digital Universidad Católica de Temuco
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Magnotti, C. ,  Figueroa Villalobos, Elías ,  Farias, J. G. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Oliveira, R. P. S. ,  Cerqueira, V. ,  [Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions] ,  Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Article

Autor: Contreras, P. Ulloa, P. Merino, O. Valdebenito Isler, Iván Figueroa Villalobos, Elías Farias, J. Risopatron, J.

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3211

Repositorio Digital Universidad Católica de Temuco
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Contreras, P. ,  Ulloa, P. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Figueroa Villalobos, Elías ,  Farias, J. ,  Risopatron, J. ,  [Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm] ,  Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
Article

Autor: Trigo, P. Merino, O. Figueroa Villalobos, Elías Valdebenito Isler, Iván Sanchez, R. Risopatron, J.

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3048

Repositorio Digital Universidad Católica de Temuco
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Trigo, P. ,  Merino, O. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Sanchez, R. ,  Risopatron, J. ,  [Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry] ,  Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
Article

Autor: Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J.

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3357

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Figueroa Villalobos, Elías ,  Cheuqueman, C. ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  Isachenko, E. ,  Sanchez, R. ,  Farias, J. ,  Risopatron, J. ,  [Short-term storage of salmonids semen in a sodium alginate-based extender] ,  Short-term storage of salmonids semen in a sodium alginate-based extender
Article

Autor: Figueroa Villalobos, Elías Merino, O. Risopatron, J. Isachenko, V. Sanchez, R. Effer Roldan, Brian Isachenko, E. Farias, J. G. Valdebenito Isler, Iván

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3702

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Figueroa Villalobos, Elías ,  Merino, O. ,  Risopatron, J. ,  Isachenko, V. ,  Sanchez, R. ,  Effer Roldan, Brian ,  Isachenko, E. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification] ,  Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

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