The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...
Merino, O.
,
Risopatrón, J.
,
Sánchez, R.
,
Isachenko, E.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]
,
Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Merino, O.
,
Ubilla Madrid, Andrea
,
Risopatron, J.
,
Farias, J. G.
,
[Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology]
,
Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...
Risopatron, J.
,
Merino, O.
,
Cheuqueman, C.
,
Figueroa Villalobos, Elías
,
Sanchez, R.
,
Farias, J. G.
,
Valdebenito Isler, Iván
,
[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]
,
Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...
Risopatrón, J.
,
Merino, O.
,
Cheuqueman, C.
,
Figueroa, E.
,
Sánchez, R.
,
Farías, Jorge G.
,
Valdebenito, I.
,
[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]
,
Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L−1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...
Magnotti, C.
,
Figueroa Villalobos, Elías
,
Farias, J.G.
,
Merino, O.
,
Valdebenito Isler, Iván
,
Oliveira, R.P.S.
,
Cerqueira, V.
,
[Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions]
,
Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...
Figueroa Villalobos, Elías
,
Risopatron, J.
,
Sanchez, R.
,
Isachenko, E.
,
Merino, O.
,
Isachenko, V.
,
Valdebenito Isler, Iván
,
[Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters]
,
Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...
Merino, O.
,
Sanchez, R.
,
Risopatron, J.
,
Isachenko, E.
,
Katkov, I. I.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Mallmann, P.
,
Isachenko, V.
,
[Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report]
,
Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report
The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...
Merino, O.
,
Risopatron, J.
,
Sanchez, R.
,
Isachenko, E.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]
,
Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...
Magnotti, C.
,
Figueroa Villalobos, Elías
,
Farias, J. G.
,
Merino, O.
,
Valdebenito Isler, Iván
,
Oliveira, R. P. S.
,
Cerqueira, V.
,
[Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions]
,
Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...
Contreras, P.
,
Ulloa, P.
,
Merino, O.
,
Valdebenito Isler, Iván
,
Figueroa Villalobos, Elías
,
Farias, J.
,
Risopatron, J.
,
[Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm]
,
Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm