The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...
Trigo, P.
,
Merino, O.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Sanchez, R.
,
Risopatron, J.
,
[Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry]
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Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...
Merino, O.
,
Figueroa Villalobos, Elías
,
Cheuqueman, C.
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
Isachenko, E.
,
Sanchez, R.
,
Farias, J.
,
Risopatron, J.
,
[Short-term storage of salmonids semen in a sodium alginate-based extender]
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Short-term storage of salmonids semen in a sodium alginate-based extender
This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....
Figueroa Villalobos, Elías
,
Merino, O.
,
Risopatron, J.
,
Isachenko, V.
,
Sanchez, R.
,
Effer Roldan, Brian
,
Isachenko, E.
,
Farias, J. G.
,
Valdebenito Isler, Iván
,
[Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification]
,
Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification