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Journal Article 2009

A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

Autor: Narina, S.S. Mohammed, I. Asiedu, Robert Mignouna, H.D.

For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...

Enlace original: https://cgspace.cgiar.org/handle/10568/90739

Investigación en Agricultura

YAMS

GENOTYPES

COLLETOTRICHUM GLOEOSPORIOIDES

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Narina, S.S. , Mohammed, I. , Asiedu, Robert , Mignouna, H.D. , [A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction] , A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

Journal Article 2003-06

A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation

Autor: Mignouna, H.D. Abang, Mathew M. Fagbemi, S.A.

Several DNA-based marker systems are available for genetic fingerprinting of plants but information on their relative usefulness for yam germplasm characterisation is lacking. The efficiency of RAPD, AFLP and SSR markers for the assessment of genetic relationships, and for cultivar identification...

Enlace original: https://cgspace.cgiar.org/handle/10568/96346

Investigación en Agricultura

DIOSCOREA ROTUNDATA

GENETIC VARIATION

GENOTYPES

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Mignouna, H.D. , Abang, Mathew M. , Fagbemi, S.A. , [A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation] , A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation

Journal Article 2009

Producing yam (Dioscorea) seeds through controlled crosses

Autor: Mignouna, H.D. Abang, Mathew M. Asiedu, Robert Geeta, R.

This protocol describes how to produce yam (Dioscorea) seeds through controlled pollination of flowers on female plants. Pollination by flower thrips takes place naturally in normal yam-growing environments in the presence of flowering male and female genotypes, although it is not efficient. This...

Enlace original: https://cgspace.cgiar.org/handle/10568/90221

Investigación en Agricultura

DIOSCOREA

GENOTYPES

POLLINATION

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Mignouna, H.D. , Abang, Mathew M. , Asiedu, Robert , Geeta, R. , [Producing yam (Dioscorea) seeds through controlled crosses] , Producing yam (Dioscorea) seeds through controlled crosses

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Resultados de búsqueda

A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation

Producing yam (Dioscorea) seeds through controlled crosses