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For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...


Narina, S.S.Mohammed, I.Asiedu, RobertMignouna, H.D.[A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction]A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced ...


Njukeng, A.P.Ariri, G.I.Hughes, Jacqueline d'ArrosAgindoran, B.O.Mignouna, H.D.Thottappilly, G.[A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.]A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.

Anthracnose, caused by Colletotrichum gloeosporioides Penz., is the most severe foliar disease of water yam (Dioscorea alata) worldwide. Population genetic analyses can yield useful insights into the evolutionary potential of C. gloeosporioides and thus lead to the development of appropriate dise...


Abang, Mathew M.Asiedu, RobertHoffmann, P.Wolf, G.A.Mignouna, H.D.Winter, S.[Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria]Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria

DNA was extracted from the mycelia of Colletotrichum gloeosporioides[Glomerella cingulata] isolates from yam, and amplification reactions using 11 random primers generated 116 DNA bands, 70 of them being informative. The electrophoretic patterns revealed by these primers allowed the identificatio...


Thottappilly, G.Mignouna, H.D.Onasanya, A.Abang, Mathew M.Oyelakin, O.Singh, N.K.[Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers]Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers

Colletotrichum gloeosporioides causes anthracnose, the most severe foliar disease of field-grown water yam (Dioscorea alata). The inheritance of resistance to a moderately virulent (FGS) strain of the pathogen was investigated in crosses between tetraploid D. alata genotypes: TDa 95/00328 (resist...


Mignouna, H.D.Abang, Mathew M.Green, K.R.Asiedu, Robert[Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)]Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)

A better knowledge of traditional cultivars is a prerequisite to overcoming the various constraints of yam production in Benin. Using IPGRI's descriptors, 560 accessions of cultivated yams (D. cayenensis Lam./D. rotundata Poir. complex) collected throughout Benin, were characterised. Ninety morph...


Dansi, A.Mignouna, H.D.Zoundjihékpon, J.Sangare, A.Asiedu, RobertQuin, F.M.[Morphological diversity, cultivar groups and possible descent in the cultivated yams (Dioscorea cayenensis/D. rotundata) complex in Benin Republic]Morphological diversity, cultivar groups and possible descent in the cultivated yams (Dioscorea cayenensis/D. rotundata) complex in Benin Republic

Based upon IBPGR (IPGRI) morphologic descriptors, 560 accessions of Benin Republic's cultivated yams (Dioscorea ccayenensis/Dioscorea rotundata complex were morphologically characterized. Ninety morphotypes were identified and accessions were classified into 26 varietal groups according to their ...


Dansi, A.Mignouna, H.D.Zoundjihékpon, J.Sangare, A.Asiedu, RobertQuinz, F.M.[Varietal identification key of the cultivated yams (Dioscorea cayenensis / Dioscorea rotundata complex) of Benin republic]Varietal identification key of the cultivated yams (Dioscorea cayenensis / Dioscorea rotundata complex) of Benin republic

Resistance to Yam mosaic virus (YMV) in tetraploid white yam (Dioscorea rotundata) is inherited differentially as a dominant and recessive character. Elite D. rotundata breeding lines with durable resistance to YMV can be developed by pyramiding major dominant and recessive genes using marker ass...


Mignouna, H.D.Abang, Mathew M.Onasanya, A.Agindotan, B.Asiedu, Robert[Identification and potential use of RAPD markers linked to yam mosaic virus resistance in white yam (Dioscorea rotundata)]Identification and potential use of RAPD markers linked to yam mosaic virus resistance in white yam (Dioscorea rotundata)

A genetic linkage map of the tetraploid water yam (Dioscorea alata L.) genome was constructed based on 469 co-dominantly scored amplified fragment length polymorphism (AFLP) markers segregating in an intraspecific F1 cross. The F1 was obtained by crossing two improved breeding lines, TDa 95/00328...


Mignouna, H.D.Mank, R.A.Ellis, T.H.N.Bosch, N.V.D.Asiedu, RobertAbang, Mathew M.Peleman, J.[A genetic linkage map of water yam (Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance]A genetic linkage map of water yam (Dioscorea alata L.) based on AFLP markers and QTL analysis for anthracnose resistance

Using IPGRI's descriptors, 45 accessions of Guinea yams (Dioscorea cayenensis Lam./ Dioscorea rotundata Poir. complex) collected in Cameroon were classified into six cultivar groups based on their morphological similarities. An identification key is proposed for the cultivar groups. Six enzymatic...


Mignouna, H.D.Dansi, A.Zok, S.[Morphological and isozymic diversity of the cultivated yams (Dioscorea cayenensis/Dioscorea rotundata complex) of Cameroon]Morphological and isozymic diversity of the cultivated yams (Dioscorea cayenensis/Dioscorea rotundata complex) of Cameroon

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