In a survey for cassava mosaic begomoviruses conducted in 1997 and 1998 in Nigeria, East African cassava mosaic virus (EACMV) was detected by the polymerase chain reaction together with African cassava mosaic virus (ACMV) in 27 out of 290 cassava leaf samples of infected plants from 254 farmers' ...
Ogbe, F.O.
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Thottappilly, G.
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Dixon, Alfred G.O.
,
Atiri, G.I.
,
Mignouna, H.D.
,
[Variants of East African cassava mosaic virus and its distribution in double infections with African cassava mosaic virus in Nigeria]
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Variants of East African cassava mosaic virus and its distribution in double infections with African cassava mosaic virus in Nigeria
Mignouna, H.D.
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Abang, Mathew M.
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Asiedu, Robert
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Geeta, R.
,
[Yam (Dioscorea) husbandry: cultivating yams in the field or greenhouse]
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Yam (Dioscorea) husbandry: cultivating yams in the field or greenhouse
For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...
Guinea yams (Dioscorea cayenensis-rotundata complex; D. rotundata Poir. and D. cayenensis Lam.) have been described as resulting from a process of domestication of wild yams of the section Enantiophyllum by African farmers. Although currently practised by few farmers, the process of yam domestica...
Mignouna, H.D.
,
Dansi, A.
,
[Yam (Dioscorea ssp.) domestication by the Nago and Fon ethnic groups in Benin]
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Yam (Dioscorea ssp.) domestication by the Nago and Fon ethnic groups in Benin
Several DNA-based marker systems are available for genetic fingerprinting of plants but information on their relative usefulness for yam germplasm characterisation is lacking. The efficiency of RAPD, AFLP and SSR markers for the assessment of genetic relationships, and for cultivar identification...
Mignouna, H.D.
,
Abang, Mathew M.
,
Fagbemi, S.A.
,
[A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation]
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A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation
A Nigerian isolate of Yam mosaic virus (YMV), genus Potyvirus from Dioscorea rotundata leaves was used to mechanically inoculate Nicotiana benthamiana (propagation host) plants from which the virus was purified. A hybridoma cell line secreting a monoclonal antibody (Mab) against YMV was produced ...
Njukeng, A.P.
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Ariri, G.I.
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Hughes, Jacqueline d'Arros
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Agindoran, B.O.
,
Mignouna, H.D.
,
Thottappilly, G.
,
[A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.]
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A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus in Dioscorea spp.
Anthracnose, caused by Colletotrichum gloeosporioides Penz., is the most severe foliar disease of water yam (Dioscorea alata) worldwide. Population genetic analyses can yield useful insights into the evolutionary potential of C. gloeosporioides and thus lead to the development of appropriate dise...
Abang, Mathew M.
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Asiedu, Robert
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Hoffmann, P.
,
Wolf, G.A.
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Mignouna, H.D.
,
Winter, S.
,
[Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria]
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Pathogenic and genetic variability among Colletotrichum gloeosporioides isolates from different yam hosts in the agroecological zones in Nigeria
DNA was extracted from the mycelia of Colletotrichum gloeosporioides[Glomerella cingulata] isolates from yam, and amplification reactions using 11 random primers generated 116 DNA bands, 70 of them being informative. The electrophoretic patterns revealed by these primers allowed the identificatio...
Thottappilly, G.
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Mignouna, H.D.
,
Onasanya, A.
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Abang, Mathew M.
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Oyelakin, O.
,
Singh, N.K.
,
[Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers]
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Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers
Colletotrichum gloeosporioides causes anthracnose, the most severe foliar disease of field-grown water yam (Dioscorea alata). The inheritance of resistance to a moderately virulent (FGS) strain of the pathogen was investigated in crosses between tetraploid D. alata genotypes: TDa 95/00328 (resist...
Mignouna, H.D.
,
Abang, Mathew M.
,
Green, K.R.
,
Asiedu, Robert
,
[Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)]
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Inheritance of resistance in water yam (Disocorea alata) to anthracnose (Colletotrichum gloeosporioides)
Alene, Arega D.
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Manyong, Victor M.
,
Omanya, G.
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Mignouna, H.D.
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Bokanga, M.
,
Odhiambo, G.
,
[Smallholder market participation under transactions costs: maize supply and fertilizer demand in Kenya]
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Smallholder market participation under transactions costs: maize supply and fertilizer demand in Kenya