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For the purpose of constructing yam cDNA libraries, attempts to isolate high quality RNA using several previously reported protocols were unsuccessful. Therefore a protocol was standardized for yam total RNA isolation by using guanidium buffer at the Department of Biology, Virginia Sate Universit...


Narina, S.S.Mohammed, I.Asiedu, RobertMignouna, H.D.[A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction]A standardized RNA isolation protocol for yam (Dioscorea alata L) cDNA library construction

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...


Abarshi, M.M.Mohammed, I.Jeremiah, S.C.Legg, J.P.Kumar, P.L.Hillocks, R.J.Maruthi, M.N.[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa

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