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Journal Article 1995

Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA

Autor: Bosompem, K.M. Moloo, S.K. Assoku, R.K.G. Nantulya, V.M.

A modified NC membrane-based dot-ELISA was used to detect and differentiate between Trypanosoma brucei, T. congolense and T. simiae procyclics in the midguts of experimentally infected tsetse flies. The modification of the assay consisted of (a) the lysis of T. congolense or T. simiae in NC membr...

Enlace original: https://cgspace.cgiar.org/handle/10568/27977

Investigación en Agricultura

GLOSSINIDAE

TRYPANOSOMES (PHYTOMONAS)

DISEASE TRANSMISSION

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Bosompem, K.M. , Moloo, S.K. , Assoku, R.K.G. , Nantulya, V.M. , [Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA] , Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA

Journal Article 2000

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Njuguna, J.T. Ndung'u, J.M.

A visually read dot-enzyme linked immunosorbent asay (dot-ELISA) developed for the detection of trypanosomes in tsetse flies (Glossina spp.) was evaluated in the laboratory and under field conditions. In the evaluation, the fly dissection method was used as a standard technique and compared to th...

Enlace original: https://cgspace.cgiar.org/handle/10568/29977

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

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Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Njuguna, J.T. , Ndung'u, J.M. , [Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)] , Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Journal Article 1998

Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

Autor: Eisler, M.C. Lessard, P. Masake, R.A. Moloo, S.K. Peregrine, A.S.

Sensitivity and specificity of the FAO/IAEA kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (three populations) or T. vivax (one population). The kits are based on mono...

Enlace original: https://cgspace.cgiar.org/handle/10568/33243

Investigación en Agricultura

CATTLE

TRYPANOSOMA CONGOLENSE

TRYPANOSOMA VIVAX

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Eisler, M.C. , Lessard, P. , Masake, R.A. , Moloo, S.K. , Peregrine, A.S. , [Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle] , Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

Journal Article 1997

Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Autor: Masake, R.A. Majiwa, P.A.O. Moloo, S.K. Makau, J.M. Njuguna, J. Maina, M. Kabata, J.M. Ole-MoiYoi, O.K. Nantulya, V.M.

The nucleic acid probes that are currently in use detect and distinguish Trypanosoma vivax parasites according to their geographic origin. To eliminate the need for using multiple DNA probes, a study was conducted to evaluate the suitability of a tandemly reiterated sequence which encodes a T. vi...

Enlace original: https://cgspace.cgiar.org/handle/10568/28613

Investigación en Agricultura

TRYPANOSOMA VIVAX

DNA

ELISA

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Masake, R.A. , Majiwa, P.A.O. , Moloo, S.K. , Makau, J.M. , Njuguna, J. , Maina, M. , Kabata, J.M. , Ole-MoiYoi, O.K. , Nantulya, V.M. , [Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction] , Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Conference Paper 1999

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

Autor: Ouma, J.O. Masake, R.A. Masiga, Daniel K. Moloo, S.K. Minja, S.H. Njuguna, J.T. Olaho-Mukani, W. Ndung'u, J.M.

Dot-enzyme linked immunosorbent assay (dot-ELISA), developed for the detection and identification of trypanosome species in tsetse flies, was validated in the laboratory by comparing its sensitivity to that of the polymerase chain reaction (PCR). Tsetse flies were experimentally infected with dif...

Enlace original: https://cgspace.cgiar.org/handle/10568/50294

Investigación en Agricultura

TRYPANOSOMIASIS

GLOSSINIDAE

ELISA

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Ouma, J.O. , Masake, R.A. , Masiga, Daniel K. , Moloo, S.K. , Minja, S.H. , Njuguna, J.T. , Olaho-Mukani, W. , Ndung'u, J.M. , [Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)] , Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)

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Resultados de búsqueda

Detection and differentiation between trypanosome species in experimentally infected tsetse flies (Glossina spp.) using dot-ELISA

Comparative sensitivity of dot-ELISA, PCR and dissection method for the detection of trypanosome infections in tsetse flies (Diptera: Glossinidae)

Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

Sensitive and specific detection of Trypanosoma vivax using the polymerase chain reaction

Comparative sensitivity of DOT - enzyme linked immunosorbent assay (DOTELISA) and the polymerase chain reaction (PCR) in the detection of trypanosome infection in tsetse flies (Glossina spp.)