Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...
Morzaria, S.P.
,
Katende, J.
,
Musoke, A.J.
,
Nene, Vishvanath
,
Skilton, Robert A.
,
Bishop, Richard P.
,
[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]
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Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa
A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...
Morzaria, S.P.
,
Dolan, T.T.
,
Norval, R.A.I.
,
Bishop, Richard P.
,
Spooner, P.R.
,
[Generation and characterisation of cloned Theileria parva parasites]
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Generation and characterisation of cloned Theileria parva parasites
Minisatellite tandem repeat elements are well known components of vertebrate genomes, but have not yet been extensively characterized in lower eukaryotes. We describe two unusual. AT-rich minisatellites of the protozoan parasite Theileria parva whose sequences are unrelated to the G/C-rich `chi m...
Bishop, Richard P.
,
Morzaria, S.P.
,
Gobright, E.I.
,
[Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva]
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Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva
Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
,
Skilton, Robert A.
,
Katende, J.
,
Wells, C.W.
,
Nene, Vishvanath
,
McElwain, T.F.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
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Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Provides a financial analysis of East Coast fever control strategies on beef production under farm conditions. Assesses the effects of immunisation by the infection and treatment method, under different acricidal treatments, on the productivity of beef cattle in Kenya.
Mukhebi, A.W.
,
Wathanga, J.
,
Perry, Brian D.
,
Irvin, A.D.
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Morzaria, S.P.
,
[Financial analysis of East Coast fever control strategies on beef production under farm conditions]
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Financial analysis of East Coast fever control strategies on beef production under farm conditions
Protein components of the cement cone of ixodid ticks are candidates for inclusion in vaccines against tick infestation, since they are essential for tick attachment and feeding. We describe here the cloning of a cDNA encoding a 36 kDa protein, designated Rhipicephalus Immuno-dominant Molecule 36...
Bishop, Richard P.
,
Lambson, B.
,
Wells, C.
,
Pandit, P.
,
Osaso, J.
,
Nkonge, C.
,
Morzaria, S.P.
,
Musoke, A.J.
,
Nene, Vishvanath
,
[A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle]
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A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle
Ngumi, P.N.
,
Lesan, S.
,
Williamson, J.R.
,
Morzaria, S.P.
,
Dolan, T.T.
,
Shaw, M.K.
,
Young, A.S.
,
[Isolation and preliminary characterization of a previously unidentified Theileria species of cattle in Kenya]
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Isolation and preliminary characterization of a previously unidentified Theileria species of cattle in Kenya
Voigt, W.P.
,
Young, A.S.
,
Mwaura, S.N.
,
Nyaga, S.G.
,
Njihia, G.M.
,
Mwakima, F.N.
,
Morzaria, S.P.
,
[In vitro feeding of instars of ixodid tick Amblyomma variegatum on skin membranes and its application to the transmission of Theileria mutans and Cowdria ruminantum]
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In vitro feeding of instars of ixodid tick Amblyomma variegatum on skin membranes and its application to the transmission of Theileria mutans and Cowdria ruminantum
Bishop, Richard P.
,
Sohanpal, B.K.
,
Kariuki, D.
,
Young, A.S.
,
Nene, Vishvanath
,
Baylis, H.
,
Allsopp, B.A.
,
Spooner, P.R.
,
Dolan, T.T.
,
Morzaria, S.P.
,
[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]
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Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction
Young, A.S.
,
Leitch, L.
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Morzaria, S.P.
,
Irvin, A.D.
,
Omwoyo, P.L.
,
Castro, J.J. de
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[Development and survival of Theileria parva parva in Rhipicephalus appendiculatus exposed in the Trans-Mara, Kenya]
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Development and survival of Theileria parva parva in Rhipicephalus appendiculatus exposed in the Trans-Mara, Kenya