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Morzaria, S.P.Young, J.R.[Restriction mapping of the genome of the protozoan parasite Theileria parva]Restriction mapping of the genome of the protozoan parasite Theileria parva

A 3-step procedure for cloning Theileria parva parasites was developed. The first step involved the in vitro infection of a fixed number of bovine lymphocytes with titrated sporozoites. The cell lines obtained from infections initiated using sporozoite/lymphocyte ratios below 1:100 were then sele...


Morzaria, S.P.Dolan, T.T.Norval, R.A.I.Bishop, Richard P.Spooner, P.R.[Generation and characterisation of cloned Theileria parva parasites]Generation and characterisation of cloned Theileria parva parasites

Minisatellite tandem repeat elements are well known components of vertebrate genomes, but have not yet been extensively characterized in lower eukaryotes. We describe two unusual. AT-rich minisatellites of the protozoan parasite Theileria parva whose sequences are unrelated to the G/C-rich `chi m...


Bishop, Richard P.Morzaria, S.P.Gobright, E.I.[Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva]Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

The relationship of strain specificity of class I major histocompatibility complex-restricted parasite-specific cytotoxic T lymphocytes (CTL) responses and cross-protection was investigated in an effort to verify the role of CTL in protection. The parasite strain specificity of the CTL responses ...


Taracha, E.L.N.Goddeeris, B.M.Morzaria, S.P.Morrison, W. Ivan[Parasite strain specificity of precursor cytotoxic T cells in individual animals correlates with cross-protection in cattle challenged with Theileria parva]Parasite strain specificity of precursor cytotoxic T cells in individual animals correlates with cross-protection in cattle challenged with Theileria parva
Fujisaki, K.Irvin, A.D.Voigt, W.P.Leitch, B.L.Morzaria, S.P.[The establishment of infection in the salivary glands of Rhipicephalus appendiculatus ticks by transplantation of kinetes of Theileria parva and the potential use of the method for parasite cloning]The establishment of infection in the salivary glands of Rhipicephalus appendiculatus ticks by transplantation of kinetes of Theileria parva and the potential use of the method for parasite cloning
Young, A.S.Morzaria, S.P.Bishop, Richard P.Dolan, T.T.Gettinby, G.[New methods of studying parasite transmission by tick]New methods of studying parasite transmission by tick

The Development of a recombinant Theileria parva vaccine is discussed under the following headings: mechanisms of protection against ECF [East Coast fever]; cloning and expression of p67; immunization and challenge of cattle; interpretation of immunization and challenge data.


Musoke, A.J.McKeever, Declan J.Morzaria, S.P.Nkonge, C.Nene, Vishvanath[A recombinant vaccine for Theileria parva]A recombinant vaccine for Theileria parva
Mwangi, D.M.Graham, S.P.Pelle, RogerHonda, Y.Tonukari, N.J.Yamage, I.M.Glew, E.J.Villiers, Etienne P. deShah, TushaarBishop, Richard P.Abuya, E.Awino, EliasGachanja, J.Luyai, A.E.Mbwika, F.Muthiani, A.M.Ndegwa, D.M.Njahira, Moses N.Nyanjui, J.K.Onono, F.O.Osaso, J.Saya, R.M.Wildmann, C.Fraser, C.M.Maudlin, I.Gardner, M.J.Morzaria, S.P.Loosmore, S.Gilbert, S.C.Audonnet, J.C.Bruggen, P. van derNene, VishvanathTaracha, E.L.N.[Exploiting host immunity and parasite genomics to develop a robust sub-unit vaccine against East Coast fever in cattle - Where are we?]Exploiting host immunity and parasite genomics to develop a robust sub-unit vaccine against East Coast fever in cattle - Where are we?

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