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Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...


Bishop, Richard P.Musoke, A.J.Morzaria, S.P.Sohanpal, B.K.Gobright, E.I.[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites
Gardner, M.J.Bishop, Richard P.Shah, TushaarVilliers, Etienne P. deCarlton, J.M.Hall, N.Ren, Q.Paulsen, I.T.Pain, A.Berriman, M.Wilson, R.J.M.Sato, S.Ralph, S.A.Mann, D.J.Xiong, Z.Shallom, S.J.Weidman, J.Jiang, L.Lynn,, J.Weaver, B.Shoaibi, A.Domingo, A.R.Wasawo, D.Crabtree, J.Wortman, J.R.Haas, B.Angiuoli, S.V.Creasy, T.H.Lu, C.Suh, B.Silva, J.C.Utterback, T.R.Feldblyum, T.V.Pertea, M.Allen, J.Nierman, W.C.Taracha, E.L.N.Salzberg, S.L.White, O.R.Fitzhugh, H.A.Morzaria, S.P.Venter, J. CraigFraser, C.M.Nene, Vishvanath[Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes]Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

Among the blood-sucking arthropods, ixodid ticks have some of the most complicated feeding behavior requirements. Feeding is preceded by an attachment process, and each instar has to remain fully attached to the same feeding site for a period of 2-14 days. These biological demands have for a long...


Young, A.S.Waladde, S.M.Morzaria, S.P.[Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission]Artificial feeding systems for ixodid ticks as a tool for study of pathogen transmission

Rhipicephalus appendiculatus adult ticks, collected int he field from zebu (Bos indicus) and exotic (Bos tarus) cattle with high antibody titres to Theileria parva schizont antigen, transmitted Theileria parva infection typical of East Coast fever to susceptible cattle. Uninfected R. appendiculat...


Kariuki, D.P.Young, A.S.Morzaria, S.P.Lesan, A.C.Mining, S.K.Omwoyo, P.Wafula, J.L.M.Molyneux, D.H.[Theileria parva carrier state in naturally infected and artificially immunized cattle]Theileria parva carrier state in naturally infected and artificially immunized cattle

Results of an experiment conducted to determine reactions following infection of cattle with Theileria parva (Boleni) challenged with T.Parva (Muguga), T.Parva (Marikebuni), T.P., Parva (Mariakani) and T.lawrencei (Nanyuti).


Irvin, A.D.Morzaria, S.P.Munatswa, F.C.Norval, R.A.I.[Immunization of cattle with a Theileria parva bovis stock from Zimbabwe protects against challenge with virulent Theileria parva parva and Theileria parva lawrencei stocks from Kenya]Immunization of cattle with a Theileria parva bovis stock from Zimbabwe protects against challenge with virulent Theileria parva parva and Theileria parva lawrencei stocks from Kenya
Morzaria, S.P.Irvin, A.D.Taracha, E.A.Spooner, P.R.Voigt, W.P.Fujinaga, T.Katende, J.M.[Immunization against East Coast fever. The use of selected stocks of Theileria parva for immunization of cattle exposed to field challenge]Immunization against East Coast fever. The use of selected stocks of Theileria parva for immunization of cattle exposed to field challenge

A large database on the transmission of a stabilate of the Theileria parva Muguga stock from one breed of cattle using two stocks of Rhipicephalus appendiculatus, Muguga and O1 Pejeta was developed and analysed. Factors associated with the ticks and cattle, and the infections developing in cattle...


Young, A.S.Dolan, T.T.Morzaria, S.P.Mwakima, F.N.Norval, R.A.I.Scott, J.Sherriff, A.Gettinby, G.[Factors influencing infections in Rhipicephalus appendiculatus ticks fed on cattle infected with Theileria parva]Factors influencing infections in Rhipicephalus appendiculatus ticks fed on cattle infected with Theileria parva

Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

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