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Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...


Morzaria, S.P.Katende, J.Musoke, A.J.Nene, VishvanathSkilton, Robert A.Bishop, Richard P.[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa

Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...


Tebele, N.Skilton, Robert A.Katende, J.Wells, C.W.Nene, VishvanathMcElwain, T.F.Morzaria, S.P.Musoke, A.J.[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Morzaria, S.P.Musoke, A.J.Latif, A.A.[Recognition of Theileria parva antigens by field sera from Rusinga Island Kenya]Recognition of Theileria parva antigens by field sera from Rusinga Island Kenya

Theileria are tick-transmitted, haemoprotozoan parasites infecting wild and domestic ungulates throughout many areas of the world. The most economically important species are T. parva and T. annulata which are pathogenic to cattle, and T. lestoquardi (syn hirci) which is pathogenic to sheep and g...


Skilton, Robert A.Musoke, A.J.Nene, VishvanathWasawo, D.P.S.Wells, C.W.Spooner, P.R.Bishop, Richard P.Osaso, J.Nkonge, C.Latif, A.Morzaria, S.P.[Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen]Molecular characterisation of a Theileria lestoquardi gene encoding a candidate sporozoite vaccine antigen

Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...


Hall, R.Boulter, N.R.Brown, C.G.D.Wilkie, G.Kirvar, E.Nene, VishvanathMusoke, A.J.Glass, E.J.Morzaria, S.P.[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva

Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...


Honda, Y.Matsubara, Y.Morzaria, S.P.McKeever, Declan J.[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes

Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...


Ballingall, K.T.Luyai, A.Rowlands, G.J.Sales, J.Musoke, A.J.Morzaria, S.P.McKeever, Declan J.[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen
Katende, J.M.Goddeeris, M.Morzaria, S.P.Nkonge, C.G.[Identification of Theileria mutans-specific antigen for use in antibody and antigen detection ELISA]Identification of Theileria mutans-specific antigen for use in antibody and antigen detection ELISA
Shapiro, S.Z.Fujisaki, K.Morzaria, S.P.Webster, P.Fujinaga, T.Spooner, P.R.Irvin, A.D.[A life-cycle stage-specific antigen of Theileria parva recognized by anti-macroschizont monoclonal antibodies]A life-cycle stage-specific antigen of Theileria parva recognized by anti-macroschizont monoclonal antibodies

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