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Fawcett, D.W.Grootenhuis, J.G.Morzaria, S.P.Conrad, P.A.[Ultrastructure of the intraerythrocytic stage of theileria species from cattle and waterbuck]Ultrastructure of the intraerythrocytic stage of theileria species from cattle and waterbuck

An improved Theileria parva DNA detection assay based on the polymerase chain reaction (PCR) using primers derived from the 104 kDa antigen (p104) gene was developed to detect parasite DNA in blood spots on filter paper. The specificity of the assay was validated using DNA from a wide range of ca...


Skilton, Robert A.Bishop, Richard P.Katende, J.M.Mwaura, S.Morzaria, S.P.[The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay]The persistence of Theileria parva infection in cattle immunised using two stocks which differ in their ability to induce a carrier state: Analysis using a novel blood spot PCR assay

Tick-borne diseases (TBDs) are a major economic constraint to livestock production in sub-Saharan Africa. ILRI is focussing on developing a range of products, such as vaccines, diagnostics and decision support services to underpin improved control programmes against these diseases. We have develo...


Morzaria, S.P.Katende, J.Musoke, A.J.Nene, VishvanathSkilton, Robert A.Bishop, Richard P.[Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa]Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens of cattle in Africa
Ngumi, P.N.Lesan, S.Williamson, J.R.Morzaria, S.P.Dolan, T.T.Shaw, M.K.Young, A.S.[Isolation and preliminary characterization of a previously unidentified Theileria species of cattle in Kenya]Isolation and preliminary characterization of a previously unidentified Theileria species of cattle in Kenya
Bishop, Richard P.Sohanpal, B.K.Kariuki, D.Young, A.S.Nene, VishvanathBaylis, H.Allsopp, B.A.Spooner, P.R.Dolan, T.T.Morzaria, S.P.[Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction]Detection of a carrier state in Theileria parva infected cattle using the polymerase chain reaction

A lambda gtll cDNA library was constructed using mRNA isolated from Theileria parva-infected bovine lymphocytes. Sequencing of random clones of this library resulted in the identification of a cDNA encoding bovine interleukin-16 (IL-16). The cDNA has an open reading frame of 1134 bp, and a 3? unt...


Mertens, B.Bishop, Richard P.Taracha, E.A.Morzaria, S.P.Nene, VishvanathSavadye, D.[Cloning, sequence and mRNA expression of bovine interleukin-16]Cloning, sequence and mRNA expression of bovine interleukin-16

Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...


Katende, J.Morzaria, S.P.Toye, Philip G.Skilton, Robert A.Nene, VishvanathNkonge, C.Musoke, A.J.[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule

The 'Muguga cocktail' which is composed of three Theileria parva stocks Muguga. Kiambu 5 and Serengeti-transformed has been used extensively for live vaccination against East Coast fever in cattle in eastern, central and southern Africa. Herein we describe the molecular characterization of the T....


Bishop, Richard P.Geysen, D.Spooner, P.R.Skilton, Robert A.Nene, VishvanathDolan, T.T.Morzaria, S.P.[Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle]Molecular and immunological characterisation of Theileria parva stocks which are components of the Muguga cocktail used for vaccination against East Coast fever in cattle

Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...


Honda, Y.Matsubara, Y.Morzaria, S.P.McKeever, Declan J.[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes

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