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The relative DNA levels of different development stages of Theileria annulata and T. parva in the cow and the tick were measured by the cytophotometric DNA technique using the fluorochrome Hoechst 33258 as a staining dye. The results revealed that sporozoites, merozoites, gamonts, and gametes wer...


Gauer, M.Mackenstedt, U.Mehlhorn, H.Schein, E.Zapf, F.Njenga, E.Young, A.Morzaria, S.P.[DNA measurements and ploidy determination of developmental stages in the life cycles of Theileria annulata and T. parva]DNA measurements and ploidy determination of developmental stages in the life cycles of Theileria annulata and T. parva

Theileria parva is the causal agent of East Coast fever (ECF), a fatal disease of cattle characterized by pyrexia, transient lymphadenopathy and panleukopenia. We have evaluated monoclonal antibodies (mAb) against three distinct antigens (p67, PIM and p32) of the parasite as immunohistological re...


Honda, Y.Matsubara, Y.Morzaria, S.P.McKeever, Declan J.[Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes]Immunohistochemical detection of parasite antigens in Theileria parva-infected bovine lymphocytes

Improvement of the livestock industry in many developing countries has been severely constrained by tick-borne diseases. The major protozoan pathogens responsible for these diseases in cattle are Theileria parva and B. annulata and Babesia bigemina and B. bovis. East Coast fever is of great econo...


Musoke, A.J.Morzaria, S.P.Nene, Vishvanath[Subunit vaccines for the control of Theileria parva]Subunit vaccines for the control of Theileria parva

The primary goals of applying molecular tools, developed at ILRI in conjunction with the use of infection and treatment immunisation are quality control of vaccines; monitoring of possible vaccine breakthrough and breakdown; and assessment of the impact of introducing new parasite types on long-t...


Bishop, Richard P.Nene, VishvanathSpooner, P.R.Mbogo, S.Kariuki, D.Payne, R.Morzaria, S.P.[Application of molecular tools in support of deployment of Theileria parva live vaccines]Application of molecular tools in support of deployment of Theileria parva live vaccines

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