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Nuclear extrachromosomal DNA elements have been identified in several kinetoplastids such as Leishmania and Trypanosoma cruzi, but never in Trypanosoma brucei. They can occur naturally or arise spontaneously as the result of sublethal drug exposure of parasites. In most cases, they are represente...


Alsford, N.S.Navarro, M.Jamnadass, Ramni H.Dunbar, H.Ackroyd, M.Murphy, N.B.Gull, K.Ersfeld, K.[The identification of circular extrachromosomal DNA in the nuclear genome of Trypanosoma brucei]The identification of circular extrachromosomal DNA in the nuclear genome of Trypanosoma brucei

Bloodstream forms (BSF) and procyclic culture forms (PCF) of African trypanosomes were incubated with a variety of lectins in vitro. Cessation of cell division and profound morphological changes were seen in procyclic forms but not in BSF after incubation with concanvavalin A (Con A). Wheat germ...


Pearson, T.W.Beecroft, R.P.Welburn, S.C.Ruepp, S.Roditi, I.Kuo-Yuan, H.Englund, P.T.Wells, C.W.Murphy, N.B.[The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro]The major cell surface glycoprotein procycline is a receptor for induction of a novel form of cell death in African trypanosomes in vitro
Alexandre, S.Guyaux, M.Murphy, N.B.Coquelet, H.Pays, A.Steinert, M.Pays, E.[Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei]Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei
Mamman, M.Gettinby, G.Murphy, N.B.Kemei, S.Peregrine, A.S.[Frequency of diminazene-resistant trypanosomes in populations of Trypanosoma congolense arising in infected animals following treatment with diminazene]Frequency of diminazene-resistant trypanosomes in populations of Trypanosoma congolense arising in infected animals following treatment with diminazene

Peregrine, A.S.Sutherland, I.A.Mutharia, L.Jamnadass, Ramni H.Gray, M.A.Codjia, V.Aliu, Y.O.Mamman, M.Silayo, R.D'Ieteren, G.D.M.Moloo, S.K.Murphy, N.B.[Overview of the chemotherapy research program at ILRAD]Overview of the chemotherapy research program at ILRAD
Dirie, M.F.Murphy, N.B.Gardiner, Peter R.[DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships]DNA fingerprinting of Trypanosoma vivax isolates rapidly identifies intraspecific relationships

Procyclic Trypanosoma brucei rhodesiense have a cell death mechanism which can be activated by an external signal, the lectin ConA, in vitro. ConA has been shown to cause profound changes in cellular morphology and induce fragmentation of nuclear DNA in T.b. rhodesiense which are characteristic o...


Murphy, N.B.Welburn, S.C.[Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs]Programmed cell death in procyclic Trypanosoma brucei rhodesiense is associated with differential expression of mRNAs
Pelle, RogerMurphy, N.B.[In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA]In vivo UV cross-linking hybridization, a powerful technique for isolating RNA binding proteins: Application to trypanosome mini-exon derived RNA

A study was carried out to determine whether a drug-resistant trypanosome population could influence the survival of a drug-sensitive population in mixed infections in goats. To identify both populations during the course of a mixed infection, a system for distinguishing them was developed; using...


Burudi, E.M.E.Peregrine, A.S.Majiwa, P.A.O.Mbiuki, S.M.Murphy, N.B.[Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats]Response of diminazene-resistance and diminazene-susceptible Trypanosoma congolense to treatment with diminazene when occuring as a mixed infection in goats

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