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Shitakha, V.M.Nantulya, V.M.Musoke, A.J.Ramasamy, R.Buscher, G.[Complement activation and fibrinolysis during infection with Theileria parva (East Coast fever) in cattle]Complement activation and fibrinolysis during infection with Theileria parva (East Coast fever) in cattle

Theileria annulata and Theileria parva both poses a major surface antigen on the sporozoite stage of the life-cycle, called SPAG-1 and p67, respectively. In each case, these antigens are vaccine candidates and have been shown to induce a degree of homologous protection in earlier work. These anti...


Hall, R.Boulter, N.R.Brown, C.G.D.Wilkie, G.Kirvar, E.Nene, VishvanathMusoke, A.J.Glass, E.J.Morzaria, S.P.[Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva]Reciprocal cross-protection induced by sporozoite antigens SPAG-1 from Theileria annulata and p67 from Theileria parva

Theileria parva is an intracellular sporozoan parasite that infects and transforms bovine lymphocytes, causing a severe lymphoproliferative disease known as East Coast fever in eastern, central and southern Africa. In this article, Declan McKeever and colleagues summarize the current understandin...


McKeever, Declan J.Taracha, E.L.N.Morrison, W. IvanMusoke, A.J.Morzaria, S.P.[Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies]Protective immune mechanisms against Theileria parva: Evolution of vaccine development strategies

Full-length recombinant versions of p67, the 709 amino acid major surface protein of Theileria parva sporozoites, induce immunity to East Coast fever (ECF) in cattle. We show that a soluble Escherichia coli recombinant version of p67 (p67635), in which a prokaryotic signal peptide replaces the e...


Bishop, Richard P.Nene, VishvanathStaeyert, J.Rowlands, G.J.Nyanjui, J.Osaso, J.Morzaria, S.P.Musoke, A.J.[Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites]Immunity to East Coast fever in cattle induced by a polypeptide fragment of the major surface coat protein of Theileria parva sporozoites

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and th...


Kaba, S.A.Musoke, A.J.Schaap, D.Schetters, T.Rowlands, G.J.Vermeulen, A.N.Nene, VishvanathVlak, J.M.Oers, M.M. van[Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle]Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as f...


Kaba, S.A.Schaap, D.Roode, E.C.Nene, VishvanathMusoke, A.J.Vlak, J.M.Oers, M.M. van[Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens]Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Morzaria, S.P.Spooner, P.R.Bishop, Richard P.Musoke, A.J.Young, J.R.[SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis]SfiI and NotI polymorphisms in theileria stocks detected by pulsed field gel electrophoresis

Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...


Ballingall, K.T.Luyai, A.Rowlands, G.J.Sales, J.Musoke, A.J.Morzaria, S.P.McKeever, Declan J.[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen
Nene, VishvanathIams, K.P.Musoke, A.J.[Molecular characterization of a Theileria parva surface sporozoite antigen]Molecular characterization of a Theileria parva surface sporozoite antigen

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