A flow cytometric method using anti-parasite antibodies was developed to measure binding of theileria parva sporozoites to the target bovine lymphocyte membrane. Parasite-host cell interactions could be inhibited by monoclonal antibodies to bovine MHC class I and partially by one of two antibodie...
Syfrig, J.
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Wells, C.
,
Daubenberger, C.A.
,
Musoke, A.J.
,
Naessens, Jan
,
[Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites]
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Proteolytic cleavage of surface proteins enhances susceptibility of lymphocytes to invasion by Theileria parva sporozoites
Vervoort, T.
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Barbet, F.
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Musoke, A.J.
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Magnus, E.
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Mpimbaza, G.W.N.
,
Meirvenne, N. van
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[Isotypic surface glycoproteins of trypanosomes]
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Isotypic surface glycoproteins of trypanosomes
Immunity to Theileria parva infection in cattle is often parasite stock specific. The antigenic diversity which is expressed at the schizont stage of the parasite together with a wild reservoir of the organism in buffalo has complicated the development of effective disease control by immunization...
Nene, Vishvanath
,
Musoke, A.J.
,
Gobright, E.I.
,
Morzaria, S.P.
,
[Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles]
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Conservation of the sporozoite p67 vaccine antigen in cattle-derived Theileria parva stocks with different cross-immunity profiles
Susceptible A/J and more resistant C57BL/6J mice were infected with Plasmodium chabaudi chabaudi 54X, P.c. chabaudi AS and Plasmodium chabaudi adami 408XZ. As expected, most C57BL/6J mice survived the infections with the different isolates. But in contrast to previous observations, not all A/J ...
Hernández Valladares, M.
,
Naessens, Jan
,
Nagda, S.M.
,
Musoke, A.J.
,
Rihet, P.
,
Ole-MoiYoi, O.K.
,
Iraqi, F.A.
,
[Comparison of pathology in susceptible A/J and resistant C57BL/6J mice after infection with different sub-strains of Plasmodium chabaudi]
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Comparison of pathology in susceptible A/J and resistant C57BL/6J mice after infection with different sub-strains of Plasmodium chabaudi
To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal seq...
Skilton, Robert A.
,
Bishop, Richard P.
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Wells, C.W.
,
Spooner, P.R.
,
Gobright, E.I.
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Nkonge, C.
,
Musoke, A.J.
,
Macklin, M.D.
,
Iams, K.P.
,
[Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)]
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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)
Theileria parva schizonts propagated in vitro in peripheral blood lymphocytes were purified and assayed for key enzymes of glucose and glycerol catabolism and the citric acid cycle. The activities of glycolytic enzymes were in the range of 21-100 nmol/min/mg protein. Glycerol kinase and ? -glycer...
Kiama, T.N.
,
Kiaira, J.K.
,
Konji, V.N.
,
Musoke, A.J.
,
[Enzymes of Glucose and Glycerol Catabolism in in vitro - propagated Theileria parva Schizonts]
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Enzymes of Glucose and Glycerol Catabolism in in vitro - propagated Theileria parva Schizonts
Musoke, A.J.
,
Morzaria, S.P.
,
Nkonge, C.
,
Jones, E.
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Nene, Vishvanath
,
[A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle]
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A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle
Palmer, G.H.
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Barbet, F.
,
Musoke, A.J.
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Katende, J.M.
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Rurangirwa, F.
,
Shkap, V.
,
Pipano, E.
,
Davis, W.C.
,
McGuire, T.C.
,
[Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United States]
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Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United States
The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has ...
Boulter, N.
,
Brown, D.
,
Wilkie, G.
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Williamson, S.
,
Kirvar, E.
,
Knight, P.
,
Glass, E.
,
Campbell, J.
,
Morzaria, S.P.
,
Nene, Vishvanath
,
Musoke, A.J.
,
Oliveria, C. de
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Gubbels, M.J.
,
Jongejan, F.
,
Hall, R.
,
[Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems]
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Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems
Protective immunity induced by the infective sporozoite stage of Theileria parva indicates a potential role for antibodies directed against conserved serologically reactive regions of the major sporozoite surface antigen p67 in vaccination to control the parasite. We have examined the allelic var...
Obara, I.
,
Ulrike, S.
,
Musoke, A.J.
,
Spooner, P.R.
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Jabbar, A.
,
Odongo, D.
,
Kemp, Stephen J.
,
Silva, J.C.
,
Bishop, Richard P.
,
[Molecular evolution of a central region containing B cell epitopes in the gene encoding the p67 sporozoite antigen within a field population of Theileria parva]
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Molecular evolution of a central region containing B cell epitopes in the gene encoding the p67 sporozoite antigen within a field population of Theileria parva