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Ole-MoiYoi, O.K.Nayar, A.Iams, K.P.Musoke, A.J.Yilma, T.[Molecular aspects of Theileria parva and approaches to vaccine development for animals]Molecular aspects of Theileria parva and approaches to vaccine development for animals

The polymorphic immunodominant molecule (PIM) of Theileria parva is expressed by the schizont and sporozoite stages of the parasite. We have recently cloned the cDNA encoding the PIM antigen from two stocks of the parasite: the cattle-derived T. parva (Muguga) stock and a buffalo-derived stock. T...


Toye, Philip G.Nyanjui, J.Goddeeris, B.M.Musoke, A.J.[Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva]Identification of neutralization and diagnostic epitopes on PIM, the polymorphic immunodominant molecule of Theileria parva

Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...


Toye, Philip G.Metzelaar, M.J.Wijngaard, P.L.J.Nene, VishvanathIams, K.P.Roose, J.Nyanjui, J.K.Gobright, E.I.Musoke, A.J.Clevers, H.C.[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva
Nene, VishvanathIams, K.P.Gobright, E.I.Musoke, A.J.[Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites]Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites
Iams, K.P.Hall, R.Webster, P.Musoke, A.J.[Identification of lambda/gt11 clones encoding the major antigenic determinants expressed by Theileria parva sporozoites]Identification of lambda/gt11 clones encoding the major antigenic determinants expressed by Theileria parva sporozoites

Previous data showed that six out of a group of nine cattle inoculated with NS1-p67, a recombinant form of a 67-KDa Theileria parva sporozoite surface protein, were immune to East Coast fever. This bacterialy expressed antigen encoded all 709 amino acid residues of p67 fused to the C-terminal end...


Nene, VishvanathInumaru, S.McKeever, Declan J.Morzaria, S.P.Shaw, M.Musoke, A.J.[Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle]Characterization of an insect cell-derived Theileria parva sporozoite vaccine antigen and immunogenicity in cattle

Initial laboratory trials of an experimental subunit vaccine against Theileria parva based on the 67-kDa major sporozoite surface antigen revealed a range of responses to challenge. We have analyzed convergence in seven sets of monozygotic twins which suggests that genetic factors may have an i...


Ballingall, K.T.Luyai, A.Rowlands, G.J.Sales, J.Musoke, A.J.Morzaria, S.P.McKeever, Declan J.[Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen]Bovine leukocyte antigen major histocompatibility complex class II DRB3*2703 and DRB3*1501 alleles are associated with variation in levels of protection against Theileria parva challenge following immunization with the sporozoite p67 antigen

The p67 sporozoite antigen of Theileria parva has been fused to the C-terminal secretion signal of Escherichia coli hemolysin and expressed in secreted form by attenuated Salmonella dublin areA strain SL5631. The recombinant p67 antigen was detected in the supernatant of transformed bacterial cul...


Gentschev, I.Glaser, I.Goebel, W.McKeever, Declan J.Musoke, A.J.Heussler, V.T.[Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle]Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: Secretion of the product enhances specific antibody responses in cattle

Theileria parva, a tick-transmitted protozoan parasite, infects cattle in eastern, central and southern Africa, leading to an acute, usually fatal lymphoproliferative disorder known as East Coast fever (1). It has been shown that the protective immune response against T. parva is mediated by clas...


Daubenberger, C.A.Heussler, V.Gobright, E.I.Wijngaard, P.L.J.Clevers, H.C.Wells, C.Tsuji, N.Musoke, A.J.McKeever, Declan J.[Molecular characterisation of a cognate 70 kDa heat shock protein of the protozoan parasite Theileria parva]Molecular characterisation of a cognate 70 kDa heat shock protein of the protozoan parasite Theileria parva
Iams, K.P.Young, J.R.Nene, VishvanathDesai, J.Webster, P.Ole-MoiYoi, O.K.Musoke, A.J.[Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva]Characterization of the gene encoding a 104 kilodalton microneme-rhoptry protein of Theileria parva

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