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Patel, EktaLubembe, D.Gachanja, J.Mwaura, S.Spooner, P.R.Toye, Philip G.[Extensive genotypic diversity in the live sporozoite East Coast Fever (ITM) vaccine]Extensive genotypic diversity in the live sporozoite East Coast Fever (ITM) vaccine

Rothen, J.Bishop, Richard P.Mwaura, S.Pfluger, V.Daubenberger, C.A.Olds, Cassandra L.[Mapping distributions of ticks and tick-borne diseases in Africa: Effects of climate change and the implications for human and animal health]Mapping distributions of ticks and tick-borne diseases in Africa: Effects of climate change and the implications for human and animal health

Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...


Musoke, A.J.Rowlands, G.J.Nene, VishvanathNyanjui, J.Katende, J.Spooner, P.R.Mwaura, S.Odongo, D.Nkonge, C.Mbogo, S.Bishop, Richard P.Morzaria, S.P.[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge

The preparation of Theileria parva stabilates has been described by Cunningham et al (1973). The method is detailed in the FAO report (1978) and the FAO tick and tick-borne Disease Manual (1984). Theileria parva stabilates are a source of viable sporozoites that can be used to immunise cattle by ...


Morzaria, S.P.Spooner, P.R.Bishop, Richard P.Mwaura, S.[The preparation of a composite stabilate for immunisation against East Coast fever]The preparation of a composite stabilate for immunisation against East Coast fever

There are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. East Coast fever, caused by the protist Theileria parva, kills one million cattle each year in sub-Saharan Africa, ...


Norling, M.Bishop, Richard P.Pelle, RogerWeihong QiHenson, S.Drábek, E.F.Tretina, K.Odongo, D.Mwaura, S.Njoroge, T.Bongcam-Rudloff, E.Daubenberger, C.A.Silva, J.C.[The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence]The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

Rhipicephalus appendiculatus is an important tick vector of several pathogens and parasitizes domestic and wild animals across eastern and southern Africa. However, its inherent genetic variation and population structure is poorly understood. To investigate whether mammalian host species, geograp...


Kanduma, E.G.Mwacharo, Joram M.Mwaura, S.Njuguna, J.N.Nzuki, I.Kinyanjui, P.W.Githaka, Naftaly W.Heyne, H.Hanotte, Olivier H.Skilton, Robert A.Bishop, Richard P.[Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya]Multi-locus genotyping reveals absence of genetic structure in field populations of the brown ear tick (Rhipicephalus appendiculatus) in Kenya

The current Infection and Treatment Method of vaccination against East Coast fever comprises an inoculation of live Theileria parva sporozoites and simultaneous administration of oxytetracycline. Immunization with a combination of parasite types has been shown to provide broader protection than i...


Patel, EktaLubembe, D.M.Gachanja, J.Mwaura, S.Spooner, P.R.Toye, Philip G.[Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity]Molecular characterization of live Theileria parva sporozoite vaccine stabilates reveals extensive genotypic diversity

The tick population of Africa includes several important genera belonging to the family Ixodidae. Many of these ticks are vectors of protozoan and rickettsial pathogens including Theileria parva that causes East Coast fever, a debilitating cattle disease endemic to eastern, central and southern A...


Rothen, J.Githaka, Naftaly W.Kanduma, E.G.Olds, Cassandra L.Pflueger, V.Mwaura, S.Bishop, Richard P.Daubenberger, C.A.[Matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of East African Ixodid tick species]Matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of East African Ixodid tick species

Biological differences, including vector competence for the protozoan parasite Theileria parva have been reported among populations of Rhipicephalus appendiculatus (Acari: Ixodidae) from different geographic regions. However, the genetic diversity and population structure of this important tick v...


Kanduma, E.G.Mwacharo, Joram M.Sunter, J.D.Nzuk, I.Mwaura, S.Kinyanjui, P.W.Kibe, M.Heyne, H.Hanotte, Olivier H.Skilton, Robert A.Bishop, Richard P.[Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus]Micro- and minisatellite-expressed sequence tag (EST) markers discriminate between populations of Rhipicephalus appendiculatus

Commercial vaccines based on the tick gut protein Bm86 have been successful in controlling the one-host tick Rhipicephalus (Boophilus) microplus and provide heterologous protection against certain other non-target ixodid tick species. This cross protection, however, does not extend to the three-h...


Olds, Cassandra L.Mwaura, S.Crowder, D.Odongo, D.Oers, M.M. vanOwen, J.Bishop, Richard P.Daubenberger, C.A.[Immunization of cattle with Ra86 impedes Rhipicephalus appendiculatus nymphal-to-adult molting]Immunization of cattle with Ra86 impedes Rhipicephalus appendiculatus nymphal-to-adult molting

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